AB227074

Anti-PPAR alpha 抗体 - ChIP Grade

Name: Anti-PPAR alpha 抗体 (ab227074) | Abcam
Brand: Abcam Limited
SKU: AB227074
Price: 59000 JPY
Availability: InStock
Rating: 5 (3 reviews)

Anti-PPAR alpha antibody

(3 Reviews)

(29 Publications)

Anti-PPAR alpha antibody (ab227074) is a rabbit polyclonal antibody detecting PPAR alpha in Western Blot. Suitable for Human, Rat.

- Over 10 publications

別名を表示する

NR1C1, PPAR, PPARA, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1

3 Images

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB227074)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPAR alpha antibody (ab227074) at 1/1000 dilution

Lane 1:

Non-transfected (-) unboiled HepG2 whole cell extracts at 30 µg

Lane 2:

Transfected (+) unboiled HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB227074)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPAR alpha antibody (ab227074) at 1/500 dilution

All lanes:

HeLa Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-PPAR alpha antibody (AB227074)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPAR alpha antibody (ab227074) at 1/2000 dilution

Lane 1:

Rat kidney tissue extracts at 50 µg

Lane 2:

Rat liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Human PPARA. The exact immunogen used to generate this antibody is proprietary information.

Q07869

特異性

This antibody does not cross-react with human PPAR gamma or PPAR delta.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-PPAR alpha antibody (ab227074) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Rat samples.

What is the molecular weight of PPAR alpha?
Anti-PPAR alpha (ab227074) specifically detects a band for PPAR alpha (UniProt: Q07869) at a molecular weight of 52kDa.

Trusted by the scientific community
Anti-PPAR alpha (ab227074) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16 : 0/18 : 1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.

See full target information PPARA

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5411-5427 PubMed40959276

2025

Therapeutic Activation of PPARα Inhibits Transformed Follicular Lymphoma Tumorigenesis via the FOXM1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Qin,Hui Zhou,Jie Zhou,Chendi Xie,Shuman Jia,Xingxing Yu,Yan Hong,Li Zhang,Yueting Huang,Yong Zhou,Bing Xu,Jie Zha

PPAR research 2024:4164906 PubMed39735727

2024

Clinical Relevance and Drug Modulation of PPAR Signaling Pathway in Triple-Negative Breast Cancer: A Comprehensive Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxia Zhang,Yunduo Liu,Mei Zhang,Guanjie Li,Siling Zhu,Keping Xie,Bin Xiao,Linhai Li

Life science alliance 8: PubMed39578076

2024

SPHK1/S1PR1/PPAR-α axis restores TJs between uroepithelium providing new ideas for IC/BPS treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Zhang,Qingyu Ge,Tianpeng Du,Yuhao Kuang,Zongyao Fan,Xinyi Jia,Wenjin Gu,Zhengsen Chen,Zhongqing Wei,Baixin Shen

Cardiovascular diagnosis and therapy 14:832-847 PubMed39513140

2024

The transcription factor PPARA mediates SIRT1 regulation of NCOR1 to protect damaged heart cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Fang Zhou,Yuntao Luo,Xu Deng,Xinyu Chen,Qin Yi

Acta pharmaceutica Sinica. B 14:3949-3963 PubMed39309511

2024

Schisanhenol ameliorates non-alcoholic fatty liver disease inhibiting miR-802 activation of AMPK-mediated modulation of hepatic lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Qi Xiao,Hongmei Zhao,Jianuo Zhang,Chunyan Yang,Yucen Zou,Bengang Zhang,Jiushi Liu,Haitao Sun,Haitao Liu

Rheumatology (Oxford, England) 63:2578-2589 PubMed38837706

2024

FTY720 ameliorates experimental MPO-ANCA-associated vasculitis by regulating fatty acid oxidation via the neutrophil PPARα-CPT1a pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Xue Wang,Luo-Yi Wang,Xiang-Yu Han,Su-Fang Chen,Xiao-Jing Sun,Zhi-Ying Li,Mark A Little,Ming-Hui Zhao,Min Chen

Journal of pharmaceutical analysis 14:100911 PubMed38807706

2024

Alginate oligosaccharide-mediated butyrate-HIF-1α axis improves skin aging in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Gao,Yixuan Li,Xiaoyu Wang,Fazheng Ren

Journal of orthopaedic surgery and research 19:109 PubMed38308345

2024

PDK4 inhibits osteoarthritis progression by activating the PPAR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengnan Li,Lifeng Xie,Hui Zeng,Yaohong Wu

Reproductive sciences (Thousand Oaks, Calif.) 31:1573-1585 PubMed38177949

2024

Proteomic Biomarkers of Intrahepatic Cholestasis of Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Weijian Zeng,Yanyan Hou,Wei Gu,Zheng Chen

Cells 12: PubMed37566072

2023

RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wu,Xiaobei Wang,Min Zhang,Parker Mathews,Yubin Kang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPAR alpha 抗体 - ChIP Grade

Anti-PPAR alpha antibody

Western blot - Anti-PPAR alpha antibody (AB227074)

Western blot - Anti-PPAR alpha antibody (AB227074)

Western blot - Anti-PPAR alpha antibody (AB227074)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (29)

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Abcam product promise