Anti-PP1C gamma 抗体 (ab16387)
Key features and details
- Sheep polyclonal to PP1C gamma
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PP1C gamma antibody
PP1C gamma 一次抗体 製品一覧 -
製品の詳細
Sheep polyclonal to PP1C gamma -
由来種
Sheep -
特異性
No cross reactivity with other recombinant pp1 isoforms. -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Xenopus laevis
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免疫原
Synthetic peptide:
TPPRGMITKQAKK
conjugated to KLH, corresponding to amino acids 311-323 of Human PPP1G1. -
ポジティブ･コントロール
- Recombinant Human PP1C gamma protein (ab114828) can be used as a positive control in WB.
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特記事項
A protein phosphatase is a phosphatase enzyme that removes a phosphate group from the phosphorylated amino acid residue of its substrate protein. Protein phosphorylation is one of the most common forms of reversible protein posttranslational modification (PTM), with up to 30% of all proteins being phosphorylated at any given time. Protein kinases (PKs) are the effectors of phosphorylation and catalyse the transfer of a γ-phosphate from ATP to specific amino acids on proteins. Several hundred PKs exist in mammals and are classified into distinct super-families. Proteins are phosphorylated predominantly on Ser, Thr and Tyr residues, which account for 79.3, 16.9 and 3.8% respectively of the phosphoproteome, at least in mammals. In contrast, protein phosphatases (PPs) are the primary effectors of dephosphorylation and can be grouped into three main classes based on sequence, structure and catalytic function. The largest class of PPs is the phosphoprotein phosphatase (PPP) family comprising PP1, PP2A, PP2B, PP4, PP5, PP6 and PP7, and the protein phosphatase Mg2+ or Mn2+-dependent (PPM) family, composed primarily of PP2C.
Source: The immunogen used to generate the purified antibody was a peptide conjugated to KLH corresponding to the sequence NH2-Thr-Pro-Pro-Arg-Gly-Met-Ile-Thr-Lys-Gln-Ala-Lys-Lys-COOH. This peptide antibody corresponds to C -terminal peptide of PP1 gamma 1 catalytic subunit having a MW of 37kD. The sequence used is amino acid 311-323.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Ammonium Sulphate Precipitation -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16387の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. -
配列類似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
細胞内局在
Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus speckle. Chromosome > centromere > kinetochore. Cleavage furrow. Midbody. Colocalizes with SPZ1 in the nucleus (By similarity). Rapidly exchanges between the nucleolar, nucleoplasmic and cytoplasmic compartments. Highly mobile in cells and can be relocalized through interaction with targeting subunits. In the presence of PPP1R8 relocalizes from the nucleolus to nuclear speckles. Shows a dynamic targeting to specific sites throughout the cell cycle. Highly concentrated in nucleoli of interphase cells and localizes at kinetochores early in mitosis. Relocalization to chromosome-containing regions occurs at the transition from early to late anaphase. Also accumulates at the cleavage furrow and midbody by telophase. - Information by UniProt
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参照データベース
- Entrez Gene: 282318 Cow
- Entrez Gene: 5501 Human
- Entrez Gene: 19047 Mouse
- Entrez Gene: 24669 Rat
- Omim: 176914 Human
- SwissProt: P61287 Cow
- SwissProt: P36873 Human
- SwissProt: P63087 Mouse
see all -
別名
- PP 1G antibody
- PP-1G antibody
- PP1C antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab16387 は 5 報の論文で使用されています。
- Verdugo-Sivianes EM et al. Mutation of SPINOPHILIN (PPP1R9B) found in human tumors promotes the tumorigenic and stemness properties of cells. Theranostics 11:3452-3471 (2021). PubMed: 33537097
- Seiki T et al. HPV-16 impairs the subcellular distribution and levels of expression of protein phosphatase 1? in cervical malignancy. BMC Cancer 15:230 (2015). PubMed: 25886518
- Kernohan KD et al. Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability. Hum Mol Genet 24:6293-300 (2015). WB, IP ; Human . PubMed: 26307080
- McCoy F et al. Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts. J Biol Chem 288:8838-48 (2013). Xenopus laevis . PubMed: 23400775
- Aoyama H et al. Isoform-specific roles of protein phosphatase 1 catalytic subunits in sarcoplasmic reticulum-mediated Ca(2+) cycling. Cardiovasc Res 89:79-88 (2011). PubMed: 20675715
