Anti-Polyethylene glycol 抗体 [EPR21993-154] - Mouse IgG2b (Chimeric) - BSA and Azide free
Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) - BSA and Azide free
- Recombinant
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Mouse Recombinant Monoclonal Polyethylene glycol antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Chemical samples.
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PEG
- I-ELISA
Lab
Indirect ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) - BSA and Azide free (AB275333)
This data was developed using ab275332, the same antibody clone in a different buffer formulation.
ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).
Antigen used at 0-500ng : NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.
Secondary antibody used at 1/1000 : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).
- WB
Lab
Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) - BSA and Azide free (AB275333)
This data was developed using ab275332, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (<a href='/products/primary-antibodies/polyethylene-glycol-antibody-epr21993-154-mouse-igg2b-chimeric-ab275332'>ab275332</a>) at 1/5000 dilution
All lanes:
BSA-methoxy PEG 5K, 10 ng
Secondary
Lane 1:
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Lane 2:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Observed band size: 250 kDa
false
Exposure time: 30s
関連する標識済み抗体及び組成の異なる製品 (1)
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Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric)
Reactivity data
製品の詳細
ab275333 is the carrier-free version of ab275332.
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody ab203857. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
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