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AB275332

Anti-Polyethylene glycol 抗体 [EPR21993-154] - Mouse IgG2b (Chimeric)

Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric)

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Mouse Recombinant Monoclonal Polyethylene glycol antibody. Suitable for I-ELISA, WB and reacts with Chemical samples.

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PEG

2 Images
Indirect ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)
  • I-ELISA

Lab

Indirect ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (ab275332, 50 ng/ml).

Antigen used at 0-500ng : NH2-PEG 5K-OCH3, NH2-PEG20K-NH2, PEG1000, PEG1000-BSA, mPEG800, mPEG800-BSA.

Secondary antibody used at 1/1000 : Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H&L).

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)
  • WB

Lab

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (AB275332)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) (ab275332) at 1/5000 dilution

All lanes:

BSA-methoxy PEG 5K, 10 ng

Secondary

Lane 1:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Lane 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 250 kDa

false

Exposure time: 30s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Polyethylene glycol antibody [EPR21993-154] - Mouse IgG2b (Chimeric) - BSA and Azide free

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

EPR21993-154

アイソタイプ

IgG2b

キャリアフリー

No

アプリケーション

WB, I-ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 50ng/ml.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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下記製品にもご興味をお持ちいただけるかもしれません:

AB215546

Polyethylene Glycol ELISA Kit

5

1 Reviews

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こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody ab203857. By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Preservative: 0.01% Sodium azide Constituents: 59.04% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information Polyethylene glycol
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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関連製品

Select an associated product type
Alternative Version
Primary Antibodies

AB203857

Anti-Polyethylene glycol antibody [EPR21993-154]

primary-antibodies

polyethylene-glycol-antibody-epr21993-154-ab203857

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(0 reviews)

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