AB203857

Anti-Polyethylene glycol 抗体 [EPR21993-154]

Anti-Polyethylene glycol antibody [EPR21993-154]

RabMAb
Advanced Validation
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Polyethylene glycol antibody. Suitable for FuncS, sELISA, ELISA, WB and reacts with Chemical samples. Cited in 1 publication.

別名を表示する

PEG

8 Images

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

FuncS

Unknown

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Method : APS sensor+ Antigen (methoxy PEG800 conjugated to BSA at 0.625 μg/ml) + Blocking (BSA) + Antibody (ab203857 at 2, 1, 0.5, 0.25, 0.125 μg/ml).

Results :

KD (M) = 5.81E-11

kon(1/Ms) = 2.70E+05

kon Error = 6.28E+02

kdis(1/s) = 1.57E-05

kdis Error = 4.49E-07

Full X²= 10.143

Full R²= 0.9993

Testing Biosensor : Aminopropylsilane (APS) Biosensors

Testing machine : Fortebio RED96e

FuncS

Unknown

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Method : APS sensor+ Antigen (methoxy PEG800 at 25 μg/ml) + Blocking (BSA) + Antibody (ab203857 at 0.5, 0.25, 0.125, 0.0625, 0.03125μg/ml).

Results :

KD (M) = 1.29E-10

kon(1/Ms) = 2.23E+06

kon Error = 1.45E+04

kdis(1/s) = 2.88E-04

kdis Error = 1.74E-06

Full X² = 3.475

Full R² = 0.9865

Testing Biosensor : Aminopropylsilane (APS) Biosensors

Testing machine : Fortebio RED96e

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA antigen dose-response curve using AffniPure Goat Anti-Mouse IgG (H&L) at 1 μg/ml concentration. The detector antibody was ab203857 anti-Polyethylene glycol at 1 μg/ml. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 10 min.

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA antigen dose-response curve using ab203857 at 2 μg/ml concentration. The detector antibody was a anti-Polyethylene glycol antibody (Biotin) (ab53449) at 500 ng/ml. The secondary antibody was a Peroxidase-conjugated Streptavidin at 1/1000 dilution.

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time : 2 min.

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 μg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-0.05μg/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 15 min.

sELISA

Unknown

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Antigen concentration range : 0-1000 ng/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : 3,3',5,5'-Tetramethylbenzidine (TMB).

Coloration time : 2 min.

ELISA

Unknown

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Direct ELISA antigen dose-response curve using ab203857 at 0.05 μg/ml concentration. The secondary antibody was a Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution.

Antigen concentration range : 0-0.05μg/ml.

Blocking/Diluting Buffer and concentration : 1% BSA/TBS.

Washing Buffer : 1 X TBST.

Substrate : p-nitrophenyl phosphate (PNPP).

Coloration time : 15 min.

Lab

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Exposure time : 92 seconds.

Blocking/Diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (ab203857) at 1/1000 dilution

All lanes:

BSA-methoxy PEG 5K, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21993-154

アイソタイプ

IgG

キャリアフリー

アプリケーション

WB, FuncS, ELISA, sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "FuncS-species-checked": "testedAndGuaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/100", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information Polyethylene glycol

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 11:1759-1777 PubMed30913540

2019

Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Jung Choi,Min-Hee Kang,Kwonho Hong,Jin-Hoi Kim

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Polyethylene glycol 抗体 [EPR21993-154]

Anti-Polyethylene glycol antibody [EPR21993-154]

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Functional Studies - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Sandwich ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

ELISA - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

Western blot - Anti-Polyethylene glycol antibody [EPR21993-154] (AB203857)

関連する標識済み抗体及び組成の異なる製品 (3)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise