AB205304

Anti-Poliovirus Receptor/PVR 抗体 [EPR17302]

Anti-Poliovirus Receptor/PVR antibody [EPR17302]

20ul selling size
RabMAb
Recombinant
KO Validated
詳細を見る

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal Poliovirus Receptor/PVR antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

別名を表示する

CD155, PVS, PVR, Poliovirus receptor, Nectin-like protein 5, NECL-5

8 Images

Supplier Data

Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Poliovirus Receptor/PVR was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : U-87 MG whole cell lysate 10μg (Input).
Lane 2 : ab205304 IP in U-87 MG whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

Predicted band size: 45 kDa

true

Supplier Data

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

Lane 1:

Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate deglycosylation (PNGase F) treated at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 70 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Lanes 1- 4 : Merged signal (red and green). Green - ab205304 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab205304 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab205304 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PVR knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PVR (Poliovirus Receptor) knockout HEK-293T cell line (<a href='/products/cell-lines/human-pvr-poliovirus-receptor-knockout-hek-293t-cell-line-ab266102'>ab266102</a>)

Lane 3:

Wild-type A549 cell lysate at 20 µg

Lane 4:

PVR knockout A549 cell lysate at 20 µg

Predicted band size: 45 kDa

Observed band size: 70 kDa

false

Lab

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Lanes 1 - 4 : Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cells. Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

PVR knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell line (<a href='/products/cell-lines/human-pvr-poliovirus-receptor-knockout-a549-cell-line-ab261877'>ab261877</a>)

Lane 3:

U87-MG whole cell lysate at 20 µg

Lane 4:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Predicted band size: 45 kDa

false

Supplier Data

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Lane 3:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 45 kDa

Observed band size: 70 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

All lanes:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 45 kDa

Observed band size: 70 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot : Anti-Poliovirus Receptor/PVR antibody [EPR17302] ab205304 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 70 kDa in Wild-type HEK-293T cell lysates with no signal observed at this size in PVR knockout HEK-293T cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysates at 20 µg

Lane 2:

Western blot - Human PVR (Poliovirus Receptor) knockout HEK-293T cell line (<a href='/products/cell-lines/human-pvr-poliovirus-receptor-knockout-hek-293t-cell-line-ab266102'>ab266102</a>) at 20 µg

Lane 3:

Human wild-type A549 cell line at 20 µg

Lane 4:

Western blot - Human PVR (Poliovirus Receptor) knockout A549 cell line (<a href='/products/cell-lines/human-pvr-poliovirus-receptor-knockout-a549-cell-line-ab261877'>ab261877</a>) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Supplier Data

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/4000 dilution

Lane 1:

HT1080 (Human fibrosarcoma cell line) whole cell lysate at 20 µg

Lane 2:

U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 45 kDa

Observed band size: 70 kDa

true

Exposure time: 30s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17302

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Poliovirus Receptor (PVR) also known as CD155 is a cell surface glycoprotein with a molecular mass of approximately 70 kDa. This receptor is expressed on a variety of cell types including epithelial and endothelial cells immune cells and fibroblasts. Another name for PVR is Necl-5 which falls under the nectin-like molecule family. It acts as an adhesion molecule and contributes to cellular signaling and junctional complexes. The expression of PVR is widespread across multiple tissues but it exhibits stronger expression in areas such as the skin and the gastrointestinal tract.

Biological function summary

The Poliovirus Receptor plays meaningful roles in immune response modulation and cell-cell adhesion. PVR interacts with components of the immune system and forms complexes with other proteins like CD226 and TIGIT. These interactions help regulate immune cell activities especially in the context of natural killer (NK) cells and T-cells. The CD155 protein also links to migration and proliferation processes which are essential for tissue formation and repair.

Pathways

PVR is involved in the regulation of immune and signaling pathways. It fits into pathways like NK cell activation and T-cell inhibitory signaling which are important for maintaining immune tolerance and preventing autoimmunity. In these pathways PVR interacts closely with other immunoregulatory proteins including CD226 and TIGIT. The partnership of PVR with these proteins shapes the delicate balance between immune activation and suppression demonstrating a clear role in immune homeostasis.

PVR relates to conditions such as cancer and viral infection. Its overexpression or altered signaling has been observed in several cancers where it may contribute to tumor growth and immune evasion. The interaction between PVR and its related protein TIGIT can affect antitumor immune responses complicating cancer progression. Additionally as its name suggests PVR binds to the poliovirus facilitating viral entry and spread during infection. This highlights the importance of PVR not only in pathogenic interactions but also in broader immune response contexts.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors : CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration.. (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.. (Microbial infection) Acts as a receptor for Pseudorabies virus.. (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells.

See full target information PVR

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:45 PubMed40217455

2025

Troxerutin suppresses the stemness of osteosarcoma via the CD155/SRC/β-catenin signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Junkai Chen,Hongbo Li,Qinglin Jin,Xiaoguang Li,Yiwen Zhang,Jingnan Shen,Gang Huang,Junqiang Yin,Changye Zou,Xinyu Li,Xin He,Xianbiao Xie,Tiao Lin

International journal of molecular sciences 26: PubMed39940693

2025

Establishment of a Panel of Human Cell Lines to Identify Cellular Receptors Used by to Infect Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia O Sosnovtseva,Thi Hoa Le,Dmitry S Karpov,Pavel O Vorobyev,Yana D Gumennaya,Olga N Alekseeva,Peter M Chumakov,Anastasia V Lipatova

Cell death & disease 12:585 PubMed34099637

2021

TRPM2 promotes pancreatic cancer by PKC/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Lin,Xunxia Bao,Hui Wang,Sibo Zhu,Zhongyan Liu,Quanning Chen,Kaixing Ai,Baomin Shi

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Poliovirus Receptor/PVR 抗体 [EPR17302]

Anti-Poliovirus Receptor/PVR antibody [EPR17302]

Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (AB205304)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise