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リコンビナントRabMAb

Anti-POLD1 抗体 [EPR15118] (ab186407)

Submit a review Submit a question References (13)
Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
  • Anti-POLD1 antibody [EPR15118] (ab186407)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15118] to POLD1
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-POLD1 antibody [EPR15118]
    POLD1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15118] to POLD1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • MOLT4, HeLa, Jurkat, K562, C6, Raw264.7 and PC12 cell lysates; Human cervix carcinoma and colon tissues; HeLa and K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15118

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186407の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/250.

ターゲット情報

  • 機能

    Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

  • 配列類似性

    Belongs to the DNA polymerase type-B family.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Polymerase (DNA directed) delta 1 catalytic subunit antibody
    • CDC2 antibody
    • CDC2 homolog antibody
    • CRCS10 antibody
    • DNA directed DNA polymerase delta 1 antibody
    • DNA directed polymerase delta 1 antibody
    • DNA pol delta 1 antibody
    • DNA polymerase delta catalytic subunit antibody
    • DNA polymerase subunit delta p125 antibody
    • DPOD1_HUMAN antibody
    • MDPL antibody
    • POLD antibody
    • POLD 1 antibody
    • POLD1 antibody
    • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa antibody
    • Polymerase (DNA) delta 1 catalytic subunit antibody
    • Polymerase DNA directed delta 1 catalytic subunit 125kD antibody
    • polymerase, DNA, delta antibody
    see all

画像

  • Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
    Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
    All lanes : Anti-POLD1 antibody [EPR15118] (ab186407) at 1/10000 dilution

    Lane 1 : MOLT4 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)

    ab186407 staining POLD1, catalytic subunit in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (ab186407)
    Flow Cytometry (Intracellular) - Anti-POLD1 antibody [EPR15118] (ab186407)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling POLD1, catalytic subunit (red) with purified ab186407 at a dilution of 1/250. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibody. 

     

     

  • Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
    Western blot - Anti-POLD1 antibody [EPR15118] (ab186407)
    All lanes : Anti-POLD1 antibody [EPR15118] (ab186407) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLD1 antibody [EPR15118] (ab186407)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling POLD1, catalytic subunit with ab186407 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)
    Immunocytochemistry/ Immunofluorescence - Anti-POLD1 antibody [EPR15118] (ab186407)

    Immunofluorescent analysis of acetone-fixed K562 cells labeling POLD1, catalytic subunit with ab186407 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Anti-POLD1 antibody [EPR15118] (ab186407)
    Anti-POLD1 antibody [EPR15118] (ab186407)

参考文献 (13)

ab186407 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186407 は 13 報の論文で使用されています。

  • Bonjoch L  et al. Unraveling the impact of a germline heterozygous POLD1 frameshift variant in serrated polyposis syndrome. Front Mol Biosci 10:1119900 (2023). PubMed: 36756361
  • Benitez A  et al. GEN1 promotes common fragile site expression. Cell Rep 42:112062 (2023). PubMed: 36729836
  • Polasek-Sedlackova H  et al. Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nat Commun 13:6090 (2022). PubMed: 36241664
  • Hou Y  et al. CTCF Mediates Replicative Senescence Through POLD1. Front Cell Dev Biol 9:618586 (2021). PubMed: 33692996
  • Gladys B  et al. Child to adulthood clinical description of MDPL syndrome due to a novel variant in POLD1. Eur J Med Genet 64:104333 (2021). PubMed: 34517090
View all Publications for this product

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