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リコンビナントRabMAb

Anti-POGZ 抗体 [EPR10612] (ab167408)

Reviews (3) Submit a question References (1)
Western blot - Anti-POGZ antibody [EPR10612] (ab167408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
  • Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (ab167408)
  • Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (ab167408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
  • Anti-POGZ antibody [EPR10612] (ab167408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10612] to POGZ
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-POGZ antibody [EPR10612]
    POGZ 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10612] to POGZ

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human POGZ aa 1-100.

  • ポジティブ･コントロール

    • HepG2 and SH-SY5Y cell lysates; Human cervical carcinoma and Human prostate tissues; HepG2 and SH-SY5Y cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10612

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab167408の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/5000. Predicted molecular weight: 155 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF (1)
1/100 - 1/250.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 155 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for IP.

ターゲット情報

  • 機能

    Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.

  • 配列類似性

    Contains 9 C2H2-type zinc fingers.
    Contains 1 DDE domain.
    Contains 1 HTH CENPB-type DNA-binding domain.

  • 細胞内局在

    Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3.
  • Information by UniProt

  • 参照データベース

    • 別名

      • KIAA0461 antibody
      • MGC71543 antibody
      • Pogo transposable element with ZNF domain antibody
      • POGZ antibody
      • POGZ_HUMAN antibody
      • putative protein product of Nbla00003 antibody
      • SUHW5 antibody
      • suppressor of hairy wing homolog antibody
      • Suppressor of hairy wing homolog 5 antibody
      • Zinc finger protein 280E antibody
      • Zinc finger protein 635 antibody
      • ZNF280E antibody
      • ZNF635 antibody
      • ZNF635M antibody
      see all

    画像

    • Western blot - Anti-POGZ antibody [EPR10612] (ab167408)
      Western blot - Anti-POGZ antibody [EPR10612] (ab167408)
      All lanes : Anti-POGZ antibody [EPR10612] (ab167408) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 155 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

      ab167408 showing +ve staining in Human normal colon.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

      ab167408 showing +ve staining in Human breast carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

      ab167408 showing +ve staining in Human ovarian carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (ab167408)
      Flow Cytometry (Intracellular) - Anti-POGZ antibody [EPR10612] (ab167408)

      Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells, labeling POGZ with ab167408 at 1/10 dilution (red) or a Rabbit IgG negative (green).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunocytochemistry/ Immunofluorescence - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunofluorescent staining of HepG2 cells labeling POGZ with ab167408 at 1/100 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling POGZ with ab167408 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POGZ antibody [EPR10612] (ab167408)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling POGZ with ab167408 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-POGZ antibody [EPR10612] (ab167408)
      Anti-POGZ antibody [EPR10612] (ab167408)

    参考文献 (1)

    ab167408 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab167408 は 1 報の論文で使用されています。

    • Hazelbaker DZ  et al. A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells. Sci Rep 10:635 (2020). PubMed: 31959800

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-POGZ antibody [EPR10612]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS cells)
    Specification
    U2OS cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Tamar Yulzary-Listovsky

    Verified customer

    投稿 Dec 03 2020

    Western blot abreview for Anti-POGZ antibody [EPR10612]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    293 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 24 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-POGZ antibody [EPR10612]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Age P0 brain (Subventricular zone))
    Permeabilization
    Yes - 0.05% Triton
    Specification
    Age P0 brain (Subventricular zone)
    Blocking step
    TNB Blocking Buffer from Perkin Elmer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 24 2019

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