Anti-Pofut1 抗体 (ab154051)
Key features and details
- Rabbit polyclonal to Pofut1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Pofut1 antibody
Pofut1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pofut1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Pig
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免疫原
Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Pofut1 (Uniprot ID: Q9H488).
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ポジティブ･コントロール
- HCT116, Mouse liver and HUVEC whole cell lysates; HCT116 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154051の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. -
組織特異性
Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 68 family. -
細胞内局在
Endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 360194 Cow
- Entrez Gene: 609881 Dog
- Entrez Gene: 23509 Human
- Entrez Gene: 140484 Mouse
- Entrez Gene: 397627 Pig
- Entrez Gene: 311551 Rat
- Omim: 607491 Human
- SwissProt: Q9H488 Human
see all -
別名
- FUT 12 antibody
- FUT12 antibody
- GDP fucose protein O fucosyltransferase 1 antibody
see all
画像
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Western blot - Anti-Pofut1 antibody (ab154051)Anti-Pofut1 antibody (ab154051) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 44 kDa
12% SDS PAGE -
Western blot - Anti-Pofut1 antibody (ab154051)Anti-Pofut1 antibody (ab154051) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Western blot - Anti-Pofut1 antibody (ab154051)Anti-Pofut1 antibody (ab154051) at 1/5000 dilution + HUVEC whole cell lysate at 15 µg
Predicted band size: 44 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Pofut1 antibody (ab154051)Immunofluorescent analysis of methanol-fixed HCT116 cells, labeling Pofut1 with ab154051 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154051 は 1 報の論文で使用されています。
- McMillan BJ et al. Structure of human POFUT1, its requirement in ligand-independent oncogenic Notch signaling, and functional effects of Dowling-Degos mutations. Glycobiology 27:777-786 (2017). PubMed: 28334865