Anti-PNUTS 抗体 [EPR11706] (ab173285)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11706] to PNUTS
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PNUTS antibody [EPR11706]
PNUTS 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11706] to PNUTS -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human PNUTS aa 550-650 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96QC0 -
ポジティブ・コントロール
- HeLa, HepG2 and Jurkat cell lysates; Human fetal brain lysate; Human breast and colon tissues; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11706 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
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ICC/IF |
1/100 - 1/250.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. |
ICC/IF
1/100 - 1/250. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers. -
組織特異性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Contains 1 C3H1-type zinc finger.
Contains 1 TFIIS N-terminal domain. -
翻訳後修飾
Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA. -
細胞内局在
Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 5514 Human
- Omim: 603771 Human
- SwissProt: Q96QC0 Human
- Unigene: 106019 Human
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別名
- CAT 53 antibody
- CAT53 antibody
- FB 19 antibody
see all
画像
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All lanes : Anti-PNUTS antibody [EPR11706] (ab173285) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNUTS with ab173285 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PNUTS with ab173285 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling PNUTS with ab173285 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab173285 は 1 報の論文で使用されています。
- Grelet S et al. Addendum: A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. Nat Cell Biol 19:1443 (2017). PubMed: 29184176