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リコンビナントRabMAb

Anti-PNUTS 抗体 [EPR11706] (ab173285)

Submit a review Submit a question References (1)
Western blot - Anti-PNUTS antibody [EPR11706] (ab173285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNUTS antibody [EPR11706] (ab173285)
  • Anti-PNUTS antibody [EPR11706] (ab173285)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11706] to PNUTS
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PNUTS antibody [EPR11706]
    PNUTS 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11706] to PNUTS

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, IHC-Pmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human PNUTS aa 550-650 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96QC0

  • ポジティブ･コントロール

    • HeLa, HepG2 and Jurkat cell lysates; Human fetal brain lysate; Human breast and colon tissues; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab173285の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
ICC/IF
1/100 - 1/250.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.

  • 組織特異性

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

  • 配列類似性

    Contains 1 C3H1-type zinc finger.
    Contains 1 TFIIS N-terminal domain.

  • 翻訳後修飾

    Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.

  • 細胞内局在

    Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity).
  • Information by UniProt

  • 参照データベース

    • 別名

      • CAT 53 antibody
      • CAT53 antibody
      • FB 19 antibody
      • FB19 antibody
      • FB19 protein antibody
      • HLA-C associated transcript 53 antibody
      • MHC class I region proline rich protein CAT53 antibody
      • MHC class I region proline-rich protein CAT53 antibody
      • p99 antibody
      • Phosphatase 1 nuclear targeting subunit antibody
      • Phosphatase nuclear targeting subunit antibody
      • PNUTS antibody
      • PP1 binding protein of 114 kDa antibody
      • PP1 nuclear targeting subunit antibody
      • PP1-binding protein of 114 kDa antibody
      • PP1R10 antibody
      • PP1RA_HUMAN antibody
      • ppp1r10 antibody
      • Protein FB19 antibody
      • Protein phosphatase 1 nuclear targeting subunit antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 10 antibody
      • Protein phosphatase 1 regulatory subunit 10 antibody
      • R111 antibody
      • Serine/threonine protein phosphatase 1 regulatory subunit 10 antibody
      • Serine/threonine-protein phosphatase 1 regulatory subunit 10 antibody
      see all

    画像

    • Western blot - Anti-PNUTS antibody [EPR11706] (ab173285)
      Western blot - Anti-PNUTS antibody [EPR11706] (ab173285)
      All lanes : Anti-PNUTS antibody [EPR11706] (ab173285) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 99 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNUTS with ab173285 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody [EPR11706] (ab173285)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PNUTS with ab173285 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-PNUTS antibody [EPR11706] (ab173285)
      Immunocytochemistry/ Immunofluorescence - Anti-PNUTS antibody [EPR11706] (ab173285)

      Immunofluorescent analysis of HeLa cells labeling PNUTS with ab173285 at 1/100 dilution.

    • Anti-PNUTS antibody [EPR11706] (ab173285)
      Anti-PNUTS antibody [EPR11706] (ab173285)

    参考文献 (1)

    ab173285 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab173285 は 1 報の論文で使用されています。

    • Grelet S  et al. Addendum: A regulated PNUTS mRNA to lncRNA splice switch mediates EMT and tumour progression. Nat Cell Biol 19:1443 (2017). PubMed: 29184176

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