Anti-PNUTS 抗体 (ab70247)
Key features and details
- Rabbit polyclonal to PNUTS
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PNUTS antibody
PNUTS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PNUTS -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to PNUTS aa 350-450.
Database link: Q96QC0 -
ポジティブ･コントロール
- Nuclear extract from Hela cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70247の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 48, 115 kDa (predicted molecular weight: 99 kDa).
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| IP |
Use a concentration of 1 - 10 µg/ml.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 48, 115 kDa (predicted molecular weight: 99 kDa). |
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IP
Use a concentration of 1 - 10 µg/ml. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers. -
組織特異性
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Contains 1 C3H1-type zinc finger.
Contains 1 TFIIS N-terminal domain. -
翻訳後修飾
Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA. -
細胞内局在
Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity). - Information by UniProt
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参照データベース
- Entrez Gene: 462544 Chimpanzee
- Entrez Gene: 5514 Human
- Entrez Gene: 52040 Mouse
- Entrez Gene: 711949 Rhesus monkey
- Omim: 603771 Human
- SwissProt: Q7YR38 Chimpanzee
- SwissProt: Q96QC0 Human
- SwissProt: Q80W00 Mouse
see all -
別名
- CAT 53 antibody
- CAT53 antibody
- FB 19 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody (ab70247)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PNUTS with ab70247 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-PNUTS antibody (ab70247)Lanes 1-2 : Anti-PNUTS antibody (ab70247) at 1 µg/ml
Lanes 3-4 : Anti-PNUTS antibody (ab70247) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extract from Hela cells at 15 µg
Lanes 2 & 4 : Nuclear extract from Hela cells at 45 µg
Predicted band size: 99 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-PNUTS antibody (ab70247)Immunoprecipitation/ Western Blot of PNUTS.
Sample: Nuclear extract from Hela cells lysate at 100µg for IP.
Lane 1: Another antibody which recognizes a different epitope of PNUTS.
Lane 2: Another antibody which recognizes a different epitope of PNUTS.
Lane 3: ab70247 at 1µg/100µg nuclear extract.
Lane 4: Another antibody which recognizes a different epitope of PNUTS.
Detection: Chemiluminescence with exposure times of 3 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab70247 は論文での使用が確認できていません。