AB70247

Anti-PNUTS 抗体

Name: Anti-PNUTS 抗体 (ab70247) | Abcam
Brand: Abcam Limited
SKU: AB70247
Price: 78000 JPY
Availability: InStock

Anti-PNUTS antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal PNUTS antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP1R10 aa 350-400.

別名を表示する

CAT53, FB19, PNUTS, PPP1R10, Serine/threonine-protein phosphatase 1 regulatory subunit 10, MHC class I region proline-rich protein CAT53, PP1-binding protein of 114 kDa, Phosphatase 1 nuclear targeting subunit, p99

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody (AB70247)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody (AB70247)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PNUTS with ab70247 at 1/1000 (1μg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-PNUTS antibody (AB70247)

Immunoprecipitation/ Western Blot of PNUTS. Sample : Nuclear extract from Hela cells lysate at 100µg for IP. Lane 1 : Another antibody which recognizes a different epitope of PNUTS. Lane 2 : Another antibody which recognizes a different epitope of PNUTS. Lane 3 : ab70247 at 1µg/100µg nuclear extract. Lane 4 : Another antibody which recognizes a different epitope of PNUTS. Detection : Chemiluminescence with exposure times of 3 seconds.

Lane 1:

Immunoprecipitation - Anti-PNUTS antibody (<a href='/products/primary-antibodies/pnuts-antibody-ab70248'>ab70248</a>)

Lane 1:

Immunoprecipitation - Anti-PNUTS antibody (ab70247)

Predicted band size: 99 kDa

false

Unknown

Western blot - Anti-PNUTS antibody (AB70247)

Lanes 1 - 2:

Western blot - Anti-PNUTS antibody (ab70247) at 1 µg/mL

Lanes 3 - 4:

Western blot - Anti-PNUTS antibody (ab70247) at 0.2 µg/mL

Lanes 1 and 3:

Nuclear extract from Hela cells at 15 µg

Lanes 2 and 4:

Nuclear extract from Hela cells at 45 µg

Predicted band size: 99 kDa

Observed band size: 115 kDa,48 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IP, WB, IHC-P

applications

免疫原

Synthetic Peptide within Human PPP1R10 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q96QC0

特異性

We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support.

バッファー組成

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The PNUTS protein also known as 'little PNUTS' acts as a regulatory subunit in the cell. It plays a role in controlling the activity of protein phosphatase 1 (PP1) essential for various cellular processes. The PNUTS protein has a molecular mass of approximately 106 kDa. It is expressed in multiple tissues with significant presence in the brain and heart suggesting its importance in these areas.

Biological function summary

The PNUTS protein influences many cellular events as it is part of a larger complex. It controls the dephosphorylation activity of PP1 impacting processes like cell cycle regulation and chromatin structure modification. This protein contributes to the dynamic regulation of transcription DNA repair and cell survival. Its ability to interact with other proteins positions it as a versatile component in cell biology.

Pathways

The PNUTS protein interacts in several notable pathways that affect cell function. It is involved in the DNA damage response pathway where it regulates DNA repair through its interaction with components like PP1. It also plays a role in the Rb signaling pathway related to cell cycle progression linking its function to cell growth and division. Through these pathways PNUTS connects with other proteins emphasizing its versatility.

Alterations in the PNUTS protein correlate with various conditions. Its dysfunction is associated with cancer impacting cell proliferation through its role in the Rb pathway. The connection between PNUTS and neurodegenerative disorders also emerges due to its high expression in the brain. In diseases like Alzheimer's PNUTS interactions with proteins like tau influence disease progression making it a target of interest in therapeutic research.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Substrate-recognition component of the PNUTS-PP1 protein phosphatase complex, a protein phosphatase 1 (PP1) complex that promotes RNA polymerase II transcription pause-release, allowing transcription elongation (PubMed : 39603239, PubMed : 39603240). Promoter-proximal pausing by RNA polymerase II is a transcription halt following transcription initiation but prior to elongation, which acts as a checkpoint to control that transcripts are favorably configured for transcriptional elongation (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex mediates the release of RNA polymerase II from promoter-proximal region of genes by catalyzing dephosphorylation of proteins involved in transcription, such as AFF4, CDK9, MEPCE, INTS12, NCBP1, POLR2M/GDOWN1 and SUPT6H (PubMed : 39603239, PubMed : 39603240). The PNUTS-PP1 complex also regulates RNA polymerase II transcription termination by mediating dephosphorylation of SUPT5H in termination zones downstream of poly(A) sites, thereby promoting deceleration of RNA polymerase II transcription (PubMed : 31677974). PNUTS-PP1 complex is also involved in the response to replication stress by mediating dephosphorylation of POLR2A at 'Ser-5' of the CTD, promoting RNA polymerase II degradation (PubMed : 33264625). The PNUTS-PP1 complex also plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (By similarity). PNUTS-PP1 complex mediates dephosphorylation of MYC, promoting MYC stability by preventing MYC ubiquitination by the SCF(FBXW7) complex (PubMed : 30158517). In addition to acts as a substrate-recognition component, PPP1R10/PNUTS also acts as a nuclear targeting subunit for the PNUTS-PP1 complex (PubMed : 9450550). In some context, PPP1R10/PNUTS also acts as an inhibitor of protein phosphatase 1 (PP1) activity by preventing access to substrates, such as RB (PubMed : 18360108).

See full target information PPP1R10

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular pharmacology 95:120-126 PubMed30397001

2018

Cell Cycle and Apoptosis Regulator 1, CCAR1, Regulates Enhancer-Dependent Nuclear Receptor CAR Transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Kanno,Shuai Zhao,Naoya Yamashita,Nao Saito,Aoi Ujiie,Rie Iijima,Nami Kikawa,Kiyomitsu Nemoto,Yoshio Inouye

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PNUTS 抗体

Anti-PNUTS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody (AB70247)

Immunoprecipitation - Anti-PNUTS antibody (AB70247)

Western blot - Anti-PNUTS antibody (AB70247)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise