Anti-PNPT1 抗体 (ab157109)
Key features and details
- Rabbit polyclonal to PNPT1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PNPT1 antibody
PNPT1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PNPT1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Macaque monkey, Rhesus monkey, Common marmoset
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免疫原
Synthetic peptide corresponding to Human PNPT1 aa 700-800.
Database link: Q8TCS8 -
ポジティブ･コントロール
- 293T, HeLa and Jurkat whole cell lysate (ab7899).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157109の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 86 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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関連性
PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain. -
細胞内局在
Mitochondrion -
参照データベース
- Entrez Gene: 100393231 Common marmoset
- Entrez Gene: 481376 Dog
- Entrez Gene: 100052355 Horse
- Entrez Gene: 87178 Human
- Entrez Gene: 71701 Mouse
- Entrez Gene: 100642182 Pig
- Entrez Gene: 360992 Rat
- GenBank: NP_149100.2 Human
see all -
別名
- 3' 5' RNA exonuclease antibody
- COXPD13 antibody
- DFNB70 antibody
see all
画像
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Western blot - Anti-PNPT1 antibody (ab157109)All lanes : Anti-PNPT1 antibody (ab157109) at 0.4 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PNPT1 antibody (ab157109)Detection of PNPT1 by Western blot of immunoprecipitate. Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1 : IP with an alternative Rabbit Anti-PNPT1 antibody.
Lane 2 : IP with ab157109.
Lane 3 : Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157109 at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PNPT1 antibody (ab157109)
All lanes : 293T whole cell lysate
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab157109 は 3 報の論文で使用されています。
- Stefan-Lifshitz M et al. Epigenetic modulation of ß cells by interferon-a via PNPT1/mir-26a/TET2 triggers autoimmune diabetes. JCI Insight 4:N/A (2019). PubMed: 30721151
- Nejad C et al. miR-222 isoforms are differentially regulated by type-I interferon. RNA 24:332-341 (2018). PubMed: 29263133
- Li D et al. Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated ß-Catenin Stabilization. Cancer Res 78:1169-1183 (2018). PubMed: 29311158