Anti-PNMT 抗体 [EPR7547(2)] (ab167427)
![Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.co.jp/ps/products/167/ab167427/Images/ab167427-165913-anti-pnmt-antibody-epr75472-western-blot.jpg "Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7547(2)] to PNMT
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PNMT antibody [EPR7547(2)]
PNMT 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7547(2)] to PNMT -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Recombinant Human PNMT protein (ab89368), K562 cell lysate and human adrenal gland and mouse kidney tissue lysates. ICC/IF: K562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR7547(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167427の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
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| ICC/IF |
1/50 - 1/100.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
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ICC/IF
1/50 - 1/100. |
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.
ターゲット情報
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機能
Converts noradrenaline to adrenaline. -
パスウェイ
Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1. -
配列類似性
Belongs to the NNMT/PNMT/TEMT family. - Information by UniProt
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参照データベース
- Entrez Gene: 5409 Human
- Entrez Gene: 18948 Mouse
- Omim: 171190 Human
- SwissProt: P11086 Human
- SwissProt: P40935 Mouse
- Unigene: 1892 Human
- Unigene: 57030 Mouse
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別名
- Noradrenaline N-methyltransferase antibody
- PENT antibody
- Phenylethanolamine N-methyltransferase antibody
see all
画像
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Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human adrenal gland lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.co.jp/ps/products/167/ab167427/Images/ab167427-165912-anti-pnmt-antibody-epr75472-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution. -
![Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.co.jp/ps/products/167/ab167427/Images/ab167427-296646-anti-pnmt-antibody-epr75472-western-blot.jpg)
Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.co.jp/ps/products/167/ab167427/Images/ab167427-4-benefits-of-recombinant-antibodies.png)
Anti-PNMT antibody [EPR7547(2)] (ab167427)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab167427 は 5 報の論文で使用されています。
- Liu C et al. Nerve growth factor causes epinephrine release dysfunction by regulating phenotype alterations and the function of adrenal medullary chromaffin cells in mice with allergic rhinitis. Mol Med Rep 27:N/A (2023). PubMed: 36601769
- Abid K et al. The noradrenergic profile of plasma metanephrine in neuroblastoma patients is reproduced in xenograft mice models and arise from PNMT downregulation. Oncotarget 12:49-60 (2021). PubMed: 33456713
- Scott AL et al. Regulation of catecholamine release from the adrenal medulla is altered in deer mice (Peromyscus maniculatus) native to high altitudes. Am J Physiol Regul Integr Comp Physiol 317:R407-R417 (2019). PubMed: 31242021
- Ni H et al. Three-dimensional image reconstruction of distribution of Pnmt+cell-derived cells in murine heart. Sci Data 4:170134 (2017). IHC-Fr ; Mouse . PubMed: 28949324
- Mahata SK et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). PubMed: 27402067