Anti-PNMT 抗体 (ab154282)
Key features and details
- Rabbit polyclonal to PNMT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-PNMT antibody
PNMT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PNMT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig
-
免疫原
Recombinant fragment corresponding to a region within amino acids 50 and 282 of Human PNMT (Uniprot ID P11086).
-
ポジティブ･コントロール
- Recombinant Human PNMT protein (ab89368) can be used as a positive control in WB. HepG2 cell lysates and U87 xenograft.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154282の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
ターゲット情報
-
機能
Converts noradrenaline to adrenaline. -
パスウェイ
Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1. -
配列類似性
Belongs to the NNMT/PNMT/TEMT family. - Information by UniProt
-
参照データベース
- Entrez Gene: 5409 Human
- Entrez Gene: 18948 Mouse
- Entrez Gene: 24661 Rat
- Omim: 171190 Human
- SwissProt: P11086 Human
- SwissProt: P40935 Mouse
- SwissProt: P10937 Rat
- Unigene: 1892 Human
see all -
別名
- Noradrenaline N-methyltransferase antibody
- PENT antibody
- Phenylethanolamine N-methyltransferase antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNMT antibody (ab154282)Immunohistochemical analysis of formalin-fixed, paraffin-embedded U87 xenograft labeling PNMT with ab154282 at 1/500.
-
Western blot - Anti-PNMT antibody (ab154282)Anti-PNMT antibody (ab154282) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 31 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab154282 は 1 報の論文で使用されています。
- Albrecht H et al. The Beta 2 Adrenergic Receptor Antagonist Timolol Improves Healing of Combined Burn and Radiation Wounds. Radiat Res 189:441-445 (2018). PubMed: 29373090