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リコンビナントRabMAb

Anti-PNK/PNKP 抗体 [EPR11935] - C-terminal (ab181107)

Reviews (2) Submit a question References (2)
Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
  • Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
  • Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
  • Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11935] to PNK/PNKP - C-terminal
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PNK/PNKP antibody [EPR11935] - C-terminal
    PNK/PNKP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11935] to PNK/PNKP - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, PC-3, Jurkat and Human testis lysate. Human breast and pancreas tissue. HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11935

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181107の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
IP (1)
1/20.
特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
IP
1/20.

ターゲット情報

  • 機能

    Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.

  • 組織特異性

    Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).

  • 関連疾患

    Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.

  • 配列類似性

    In the N-terminal section; belongs to the DNA 3' phosphatase family.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 2''(3'')-polynucleotidase antibody
      • 2'(3')-polynucleotidase antibody
      • Bifunctional polynucleotide phosphatase/kinase antibody
      • DEM 1 antibody
      • DEM1 antibody
      • DNA 5' kinase/3' phosphatase antibody
      • DNA 5''-kinase/3''-phosphatase antibody
      • EIEE10 antibody
      • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
      • MCSZ antibody
      • PNK 1 antibody
      • PNK1 antibody
      • Pnkp antibody
      • PNKP DNA kinase antibody
      • PNKP_HUMAN antibody
      • Polynucleotide 3'-phosphatase antibody
      • Polynucleotide 5' hydroxyl kinase antibody
      • Polynucleotide 5''-hydroxyl-kinase antibody
      • Polynucleotide kinase 3 prime phosphatase antibody
      • Polynucleotide kinase 3' phosphatase antibody
      • Polynucleotide Kinase antibody
      • Polynucleotide kinase-3''-phosphatase antibody
      see all

    画像

    • Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 57 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK/PNKP with ab181107 at 1/100 dilution.  Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      Western blot - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : PC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 57 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      Immunoprecipitation - Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

      Western blot analysis of PNK/PNKP in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.

    • Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)
      Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107)

    参考文献 (2)

    ab181107 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab181107 は 2 報の論文で使用されています。

    • Kalasova I  et al. Pathological mutations in PNKP trigger defects in DNA single-strand break repair but not DNA double-strand break repair. Nucleic Acids Res N/A:N/A (2020). PubMed: 32504494
    • Yang B  et al. The lipogenic LXR-SREBF1 signaling pathway controls cancer cell DNA repair and apoptosis and is a vulnerable point of malignant tumors for cancer therapy. Cell Death Differ 27:2433-2450 (2020). PubMed: 32144382

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PNK/PNKP antibody [EPR11935] - C-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Gradient Gel)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Blocking step
    Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 15 2018

    Immunoprecipitation abreview for Anti-PNK antibody [EPR11935] - C-terminal

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Total protein in input
    500 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    U2OS cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 15 2018

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