Anti-PNAD 抗体 (ab111710)
Key features and details
- Rabbit polyclonal to PNAD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PNAD antibody
PNAD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PNAD -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Pig
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免疫原
Recombinant fragment corresponding to Human PNAD aa 1-246.
Database link: AAH17336 -
ポジティブ･コントロール
- 293T and Raji whole cell lysates; Human hepatoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab111710 is purified by antigen-affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) |
ターゲット情報
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関連性
PNAD is required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 123803 Human
- Entrez Gene: 18203 Mouse
- Entrez Gene: 397107 Pig
- Entrez Gene: 360462 Rat
- SwissProt: Q96AB6 Human
- SwissProt: Q64311 Mouse
- SwissProt: Q28955 Pig
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別名
- N terminal Asn amidase antibody
- N terminal asparagine amidase antibody
- N terminal asparagine amidohydrolase antibody
see all
画像
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Western blot - Anti-PNAD antibody (ab111710)Anti-PNAD antibody (ab111710) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS-PAGE. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNAD antibody (ab111710)ab111710 at 1/500 dilution staining PNAD in Formalin-fixed, paraffin-embedded Human Hepatoma tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab111710 は論文での使用が確認できていません。