Anti-PMP70 抗体 (ab85550)
Key features and details
- Rabbit polyclonal to PMP70
- Suitable for: IHC-P, ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PMP70 antibody
PMP70 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PMP70 -
由来種
Rabbit -
特異性
Replenishment batches of our polyclonal antibody, ab85550 are tested in WB. Previous batches were additionally validated in ICC/IF and IHC-P. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab109448.
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アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Orangutan
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免疫原
Synthetic peptide corresponding to Rat PMP70 aa 600 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab94821) -
ポジティブ･コントロール
- WB: Rat liver and kidney, Human liver and Mouse Lung, tissue lysates, A431, HepG2 and HeLa whole cell lysates. IHC-P: Human cervical carcinoma tissue. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85550の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa).
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 75 kDa). |
ターゲット情報
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機能
Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity. -
配列類似性
Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain. -
細胞内局在
Peroxisome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 424487 Chicken
- Entrez Gene: 526059 Cow
- Entrez Gene: 5825 Human
- Entrez Gene: 19299 Mouse
- Entrez Gene: 100172102 Orangutan
- Entrez Gene: 25270 Rat
- Omim: 170995 Human
- SwissProt: P28288 Human
see all -
別名
- 70 kDa peroxisomal membrane protein antibody
- ABC 43 antibody
- ABC D3 antibody
see all
画像
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Western blot - Anti-PMP70 antibody (ab85550)All lanes : Anti-PMP70 antibody (ab85550) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : ABCD3 (PMP70) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 75 kDaLanes 1 - 4: Merged signal (red and green). Green - ab85550 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab85550 was shown to specifically react with PMP70 in wild-type HAP1 cells as signal was lost in ABCD3 (PMP70) knockout cells. Wild-type and ABCD3 (PMP70) knockout samples were subjected to SDS-PAGE. Ab85550 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-PMP70 antibody (ab85550)ab85550 staining PMP70 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85550 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Western blot - Anti-PMP70 antibody (ab85550)All lanes : Anti-PMP70 antibody (ab85550) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Kidney (Rat) Tissue Lysate
Lane 3 : Human liver tissue lysate - total protein (ab29889)
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 28 kDa, 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
The 73 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Rat ATP-binding cassette sub-family D member 3 (PMP70). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody (ab85550)IHC image of Ab85550 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab85550, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-PMP70 antibody (ab85550)Anti-PMP70 antibody (ab85550) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab85550 は 16 報の論文で使用されています。
- Li F et al. A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry. Int J Mol Sci 23:N/A (2022). PubMed: 35563005
- Jiang S et al. Peroxisomal Fitness: A Potential Protective Mechanism of Fenofibrate against High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice. Diabetes Metab J 46:829-842 (2022). PubMed: 35746892
- Loughran PA et al. Immunohistochemistry. Curr Protoc 2:e549 (2022). PubMed: 36102926
- Sograte-Idrissi S et al. Circumvention of common labelling artefacts using secondary nanobodies. Nanoscale 12:10226-10239 (2020). PubMed: 32356544
- Demaret T et al. Accurate and live peroxisome biogenesis evaluation achieved by lentiviral expression of a green fluorescent protein fused to a peroxisome targeting signal 1. Histochem Cell Biol 153:295-306 (2020). PubMed: 32124009