AB229996

Anti-PMM2 抗体

Name: Anti-PMM2 抗体 (ab229996) | Abcam
Brand: Abcam Limited
SKU: AB229996
Price: 74000 JPY
Availability: InStock

Anti-PMM2 antibody

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(3 Publications)

Rabbit Polyclonal PMM2 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PMM2.

別名を表示する

Phosphomannomutase 2, PMM 2, PMM2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Paraffin-embedded human thyroid tissue stained for PMM2 using ab229996 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Paraffin-embedded human gastric cancer tissue stained for PMM2 using ab229996 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Immunoprecipitation - Anti-PMM2 antibody (AB229996)

PMM2 was immunoprecipitated from 500 μg HeLa (Human breast adenocarcinoma cell line) whole cell lysate using 4 μg of ab229996.

Lane 1 : Rabbit monoclonal IgG (1 μg) IP in HeLa whole cell lysate.

Lane 2 : ab229996 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 20 μg (Input).

For western blotting an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-PMM2 antibody (ab229996)

Predicted band size: 28 kDa

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Supplier Data

Western blot - Anti-PMM2 antibody (AB229996)

All lanes:

Western blot - Anti-PMM2 antibody (ab229996) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 6:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 28 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, IP

applications

免疫原

Recombinant Full Length Protein corresponding to Human PMM2.

O15305

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phosphomannomutase 2 (PMM2) also known as PMM 22 or PMM II is an enzyme that plays a role in the conversion of mannose-6-phosphate to mannose-1-phosphate. PMM2 is about 29 kDa in size and exists predominantly in the cytoplasm of cells. It shows widespread expression but higher levels are found in the liver brain and muscle tissues. PMM2 activity is important in carbohydrate metabolism making it an enzyme of interest especially in the context of glycobiology and metabolic research.

Biological function summary

PMM2 catalyzes an important step in the biosynthesis of GDP-mannose which is necessary for glycan production. These glycans are essential in the process of glycosylation a post-translational modification of proteins and lipids. PMM2 acts independently and does not form part of a larger protein complex. Through its role in glycan synthesis PMM2 impacts cell signaling protein folding and molecular interactions.

Pathways

PMM2 is involved in the N-glycosylation pathway and the GDP-mannose biosynthesis pathway. It collaborates closely with proteins such as PMM1 as both contribute to mannose metabolism and glycan assembly. PMM2 is significant in these pathways as it impacts not only the formation of sugar nucleotides but also their integration into larger biomolecules.

PMM2 dysfunction is directly linked to Congenital Disorders of Glycosylation Type Ia (CDG-Ia). This genetic disorder results from mutations in the PMM2 gene impairing glycosylation processes and resulting in multisystemic clinical manifestations. The protein PMM1 while not directly associated with the disorder serves complementary functions in mannose phosphate isomerization and may modulate the impact of PMM2 deficiencies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.

See full target information PMM2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 584:112160 PubMed38266771

2024

PMM2 controls ERα levels and cell proliferation in ESR1 Y537S variant expressing breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cipolletti,Filippo Acconcia

Frontiers in veterinary science 7:570593 PubMed33240950

2020

Effects of Probiotics BaSC06 on Intestinal Digestion and Absorption, Antioxidant Capacity, Microbiota Composition, and Macrophage Polarization in Pigs for Fattening.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefang Cao,Li Tang,Zihan Zeng,Baikui Wang,Yuanhao Zhou,Qi Wang,Peng Zou,Weifen Li

Journal of assisted reproduction and genetics 37:443-450 PubMed31902100

2020

Novel PMM2 missense mutation in a Chinese family with non-syndromic premature ovarian insufficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Tianliu Peng,Chao Lv,Hangjing Tan,Jiafeng Huang,Hailun He,Yan Wang,Minghua Zeng,Dajing Yi,Jie Li,Hongwen Deng,Xiaobo Shi,Hongmei Xiao

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PMM2 抗体

Anti-PMM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMM2 antibody (AB229996)

Immunoprecipitation - Anti-PMM2 antibody (AB229996)

Western blot - Anti-PMM2 antibody (AB229996)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise