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AB4645

Anti-Pma1 抗体 [40B7]

Anti-Pma1 antibody [40B7]

4

(1 Review)

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(32 Publications)

Mouse Monoclonal PMA1 antibody. Suitable for WB, ICC/IF and reacts with Saccharomyces cerevisiae samples. Cited in 32 publications.

別名を表示する

YGL008C, PMA1, Plasma membrane ATPase 1, Proton pump 1

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

40B7

アイソタイプ

IgG

キャリアフリー

No

交差種

Saccharomyces cerevisiae

アプリケーション

ICC/IF, WB

applications

特異性

Reacts with Pma1p based on the size of the band detected on western blots and the immunofluorescence localization pattern.

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組合せ製品

Primary Antibodies

AB9110

Anti-HA tag antibody - ChIP Grade

Immunoprecipitation - Anti-HA tag antibody - ChIP Grade (AB9110)

  • 5
  • 59
Primary Antibodies

AB113683

Anti-VPH1 antibody [10D7A7B2]

Western blot - Anti-VPH1 antibody [10D7A7B2] (AB113683)

  • 3
  • 1
Primary Antibodies

AB113684

Anti-VMA2 antibody [13D11B2]

Western blot - Anti-VMA2 antibody [13D11B2] (AB113684)

  • 0
  • 0
Primary Antibodies

AB113686

Anti-DPM1 antibody [5C5A7]

Western blot - Anti-DPM1 antibody [5C5A7] (AB113686)

  • 3
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>(cell lysates, ECL detection). For non-ECL western detection methods, 1/1000 - 1/5000. Also detects Pma1p-containing fusion proteins on western blots.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
精製に関する特記事項
Sterile filtered.
バッファー組成
Preservative: 0.065% Sodium azide Constituents: Tissue culture supernatant
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Pma1 also known as plasma membrane H+-ATPase is an essential enzyme that functions as a proton pump in cells. It maintains a proton gradient across the plasma membrane by extruding H+ ions out of the cell which is an important process for cell homeostasis. Pma1 has a molecular mass of approximately 100 kDa. It is mainly expressed in the plasma membrane of yeast cells and other fungi where it plays a critical role in regulating intracellular pH and membrane potential.
Biological function summary

The plasma membrane H+-ATPase activity of Pma1 facilitates nutrient uptake and cell growth by providing an electrochemical gradient. In yeast it forms no known complex but works in conjunction with other membrane transporters to manage ions and metabolite exchange. Through this activity Pma1 indirectly supports various cellular processes including metabolism stress responses and signal transduction.

Pathways

The proton pump action of Pma1 is integral to the regulation of osmotic balance and energy metabolism pathways. It is linked to the glycolytic pathway by influencing the uptake of glucose through transporters. Pma1 is also associated with the oxidative phosphorylation pathway by maintaining the proton motive force necessary for ATP synthesis. It interacts with proteins such as glucose transporters and ion channels to ensure proper metabolic functions.

Pma1 is linked to the understanding of fungal infections as its function is critical for fungal cell survival. Inhibiting Pma1 can be a potential therapeutic target considering its absence in mammalian cells thereby providing specificity. It also offers insight into acidophilic disorders where proton gradients are disrupted. While no direct human protein counterparts exist its functional analogy in proton pumps relates it to the broader category of proton-pump-associated conditions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H(+)-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
See full target information PMA1
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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 80:32 PubMed36609589

2023

HSP70-binding motifs function as protein quality control degrons.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda B Abildgaard,Vasileios Voutsinos,Søren D Petersen,Fia B Larsen,Caroline Kampmeyer,Kristoffer E Johansson,Amelie Stein,Tommer Ravid,Claes Andréasson,Michael K Jensen,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

The Journal of cell biology 221: PubMed36112368

2022

Triglyceride lipolysis triggers liquid crystalline phases in lipid droplets and alters the LD proteome.

Applications

Unspecified application

Species

Unspecified reactive species

Sean Rogers,Long Gui,Anastasiia Kovalenko,Valeria Zoni,Maxime Carpentier,Kamran Ramji,Kalthoum Ben Mbarek,Amelie Bacle,Patrick Fuchs,Pablo Campomanes,Evan Reetz,Natalie Ortiz Speer,Emma Reynolds,Abdou Rachid Thiam,Stefano Vanni,Daniela Nicastro,W Mike Henne

BMC biology 20:163 PubMed35840963

2022

Proton export upregulates aerobic glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Shonagh Russell,Liping Xu,Yoonseok Kam,Dominique Abrahams,Bryce Ordway,Alex S Lopez,Marilyn M Bui,Joseph Johnson,Tamir Epstein,Epifanio Ruiz,Mark C Lloyd,Pawel Swietach,Daniel Verduzco,Jonathan Wojtkowiak,Robert J Gillies

Structure (London, England : 1993) 30:1245-1253.e5 PubMed35700725

2022

Disease-linked mutations cause exposure of a protein quality control degron.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Kampmeyer,Sven Larsen-Ledet,Morten Rose Wagnkilde,Mathias Michelsen,Henriette K M Iversen,Sofie V Nielsen,Søren Lindemose,Alberto Caregnato,Tommer Ravid,Amelie Stein,Kaare Teilum,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

PLoS genetics 17:e1009539 PubMed33914734

2021

Mapping the degradation pathway of a disease-linked aspartoacylase variant.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Gersing,Yong Wang,Martin Grønbæk-Thygesen,Caroline Kampmeyer,Lene Clausen,Martin Willemoës,Claes Andréasson,Amelie Stein,Kresten Lindorff-Larsen,Rasmus Hartmann-Petersen

International journal of biological macromolecules 171:343-357 PubMed33421469

2021

Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Cátia Santos-Pereira,María T Andrés,Susana R Chaves,José F Fierro,Hernâni Gerós,Stéphen Manon,Lígia R Rodrigues,Manuela Côrte-Real

Methods in molecular biology (Clifton, N.J.) 2049:165-190 PubMed31602611

2019

Mapping the Saccharomyces cerevisiae Spatial Proteome with High Resolution Using hyperLOPIT.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J H Nightingale,Stephen G Oliver,Kathryn S Lilley

Bio-protocol 9:e3303 PubMed33654815

2019

A Protocol to Map the Spatial Proteome Using HyperLOPIT in .

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J H Nightingale,Kathryn S Lilley,Stephen G Oliver

The Journal of cell biology 218:961-976 PubMed30578283

2018

Rab5 GTPases are required for optimal TORC2 function.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa N Locke,Jeremy Thorner

Environmental science and pollution research international 26:2375-2386 PubMed30467749

2018

Polychlorinated biphenyl 126 exposure in rats alters skeletal muscle mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Tremblay-Laganière,Léa Garneau,Jean-François Mauger,Vian Peshdary,Ella Atlas,Alyssa Samantha Nikolla,Natalie Ann Chapados,Céline Aguer
View all publications
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