Anti-PLVAP/PV-1 抗体 [174/2] (ab81719)
Key features and details
- Mouse monoclonal [174/2] to PLVAP/PV-1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PLVAP/PV-1 antibody [174/2]
PLVAP/PV-1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [174/2] to PLVAP/PV-1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human tonsil cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab81719 is 0.2µM filtered. -
ポリ/モノ
モノクローナル -
クローン名
174/2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81719の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt | ||
ICC/IF |
特記事項 |
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ICC/IF: Typical starting dilution 1/50.
IHC-Fr: Typical starting dilution 1/50.
WB: Typical starting dilution 1/50. Predicted molecular weight: 50 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Involved in the formation of stomatal and fenestral diaphragms of caveolae. May function in microvascular permeability. -
組織特異性
Expressed in lung, kidney, heart, aorta, placenta, muscle, pituitary gland, adrenals, mammary gland, bladder, lymph node, bone marrow, trachea, digestive tract, liver and tumor-associated endothelium. -
細胞内局在
Cell membrane. Membrane > caveola. Cytoplasm > perinuclear region. Membrane-associated protein of caveolae. Found in fenestral and stomatal diaphragms in fenestrated endothelia and transendothelial channels. Also colocalized with CAV1 in perinuclear region. - Information by UniProt
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参照データベース
- Entrez Gene: 83483 Human
- Omim: 607647 Human
- SwissProt: Q9BX97 Human
- Unigene: 107125 Human
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別名
- FELS antibody
- Fenestrated endothelial linked structure protein antibody
- Fenestrated endothelial-linked structure protein antibody
see all
画像
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ab81719 at 1/50 dilution staining PLVAP/PV-1 in human tonsil by Immunofluorescence.
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Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab81719 は 9 報の論文で使用されています。
- Song MJ et al. Bioprinted 3D outer retina barrier uncovers RPE-dependent choroidal phenotype in advanced macular degeneration. Nat Methods 20:149-161 (2023). PubMed: 36550275
- Ren J et al. Single-cell sequencing reveals that endothelial cells, EndMT cells and mural cells contribute to the pathogenesis of cavernous malformations. Exp Mol Med 55:628-642 (2023). PubMed: 36914857
- Wilkinson AL et al. The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration. iScience 26:107966 (2023). PubMed: 37810232
- Blain R et al. A tridimensional atlas of the developing human head. Cell 186:5910-5924.e17 (2023). IHC - Wholemount ; Human . PubMed: 38070509
- Muranyi W et al. Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus. iScience 25:104383 (2022). PubMed: 35633941
- Nakagami Y et al. An anti-PLVAP antibody suppresses laser-induced choroidal neovascularization in monkeys. Eur J Pharmacol 854:240-246 (2019). PubMed: 31026444
- Belle M et al. Tridimensional Visualization and Analysis of Early Human Development. Cell 169:161-173.e12 (2017). PubMed: 28340341
- Wisniewska-Kruk J et al. Plasmalemma Vesicle-Associated Protein Has a Key Role in Blood-Retinal Barrier Loss. Am J Pathol 186:1044-54 (2016). PubMed: 26878208
- Elkadri A et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). PubMed: 26207260