Anti-PLOD2/LH2 抗体 (ab72939)
Key features and details
- Mouse polyclonal to PLOD2/LH2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PLOD2/LH2 antibody
PLOD2/LH2 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to PLOD2/LH2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human PLOD2/LH2 aa 1-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_891988.1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72939の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. -
組織特異性
Highly expressed in pancreas and muscle. Isoform 1 and isoform 2 are expressed in the majority of the examined cell types. Isoform 2 is specifically expressed in skin, lung, dura and aorta. -
関連疾患
Defects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2) [MIM:609220]. Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal. -
配列類似性
Contains 1 Fe2OG dioxygenase domain. -
細胞内局在
Rough endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5352 Human
- Omim: 601865 Human
- SwissProt: O00469 Human
- Unigene: 477866 Human
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別名
- 2-oxoglutarate 5-dioxygenase 2 antibody
- LH2 antibody
- Lysine hydroxylase 2 antibody
see all
画像
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Western blot - Anti-PLOD2/LH2 antibody (ab72939)Anti-PLOD2/LH2 antibody (ab72939) at 1 µg/ml + human kidney tissue lysate at 25 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
Western blot - Anti-PLOD2/LH2 antibody (ab72939)All lanes : Anti-PLOD2/LH2 antibody (ab72939) at 1 µg/ml
Lane 1 : PLOD2/LH2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 85 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-PLOD2/LH2 antibody (ab72939)ab72939, at 10µg/ml, staining PLOD2/LH2 in Hela cells by immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab72939 は 3 報の論文で使用されています。
- Ju HQ et al. A circRNA signature predicts postoperative recurrence in stage II/III colon cancer. EMBO Mol Med 11:e10168 (2019). PubMed: 31475771
- Kiyozumi Y et al. PLOD2 as a potential regulator of peritoneal dissemination in gastric cancer. Int J Cancer 143:1202-1211 (2018). PubMed: 29603227
- Shao S et al. Lysyl Hydroxylase Inhibition by Minoxidil Blocks Collagen Deposition and Prevents Pulmonary Fibrosis via TGF-ß1/Smad3 Signaling Pathway. Med Sci Monit 24:8592-8601 (2018). PubMed: 30481795
