Anti-Plexin D1 抗体 (ab28762)
Key features and details
- Goat polyclonal to Plexin D1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Plexin D1 antibody
Plexin D1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Plexin D1 -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human Plexin D1 aa 1683-1694.
Sequence:LAEPKKSHRQSH
Database link: Q9Y4D7 -
ポジティブ･コントロール
- Flow Cyt (Intra): K562 cells. ICC/IF: HeLa and U251 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28762の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
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| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 212 kDa.
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| IHC-P | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
|
| IP |
Use at an assay dependent concentration. PubMed: 20385769
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 212 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
IP
Use at an assay dependent concentration. PubMed: 20385769 |
ターゲット情報
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組織特異性
Detected at low levels in heart, placenta, lung, skeletal muscle, kidney, thymus and liver. Detected at very low levels in brain, colon, spleen, small intestine and peripheral blood leukocytes. -
配列類似性
Belongs to the plexin family.
Contains 3 IPT/TIG domains.
Contains 1 Sema domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23129 Human
- Entrez Gene: 67784 Mouse
- Omim: 604282 Human
- SwissProt: Q9Y4D7 Human
- SwissProt: Q3UH93 Mouse
- SwissProt: Q68HV1 Mouse
- SwissProt: Q8VCL8 Mouse
- SwissProt: Q9CZF2 Mouse
see all -
別名
- KIAA0620 antibody
- MGC75353 antibody
- PLEX D1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (ab28762)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
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Flow Cytometry (Intracellular) - Anti-Plexin D1 antibody (ab28762)Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Incubated with ab28762 for 1 hour (10 µg/ml) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml).
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-Plexin D1 antibody (ab28762)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab28762 は 11 報の論文で使用されています。
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Sun M et al. PLXND1-mediated calcium dyshomeostasis impairs endocardial endothelial autophagy in atrial fibrillation. Front Physiol 13:960480 (2022). PubMed: 36017337
- Walter LM et al. Profilin2 regulates actin rod assembly in neuronal cells. Sci Rep 11:10287 (2021). PubMed: 33986363
- Li J et al. Prognostic Value of PLXND1 and TGF-ß1 Coexpression and Its Correlation With Immune Infiltrates in Hepatocellular Carcinoma. Front Oncol 10:604131 (2020). PubMed: 33489909
- Yoshida Y et al. Peptide vaccine for semaphorin3E ameliorates systemic glucose intolerance in mice with dietary obesity. Sci Rep 9:3858 (2019). PubMed: 30846754