Name: Anti-Plectin antibody [E398P]
Brand: RabMAb
SKU: ab32528
Rating: 5 (5 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (ab32528)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E398P] to Plectin
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human

Carrier Free

製品名

Anti-Plectin antibody [E398P]
Plectin 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [E398P] to Plectin
由来種

Rabbit
特異性

This antibody recognises plectin, a 500-kDa intermediate filament binding protein.
アプリケーション

適用あり: IHC-P, WBmore details
適用なし: Flow Cyt
種交差性

交差種: Human
交差が予測される動物種: Mouse, Rat
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- Jurkat whole cell lysate (ab7899), human skeletal muscle WB: U-2 OS cell lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

E398P
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Plectin antibody [E398P] - BSA and Azide free (ab176475)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab32528の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P	(1)	1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB	(1)	1/5000. Predicted molecular weight: 532 kDa.

特記事項
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000. Predicted molecular weight: 532 kDa.

追加情報

Is unsuitable for Flow Cyt.

機能

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofibers integrity.
組織特異性

Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
関連疾患

Epidermolysis bullosa simplex with pyloric atresia
Epidermolysis bullosa simplex, with muscular dystrophy
Epidermolysis bullosa simplex, Ogna type
Limb-girdle muscular dystrophy 2Q
配列類似性

Belongs to the plakin or cytolinker family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 33 plectin repeats.
Contains 4 spectrin repeats.
ドメイン

The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N-and the C-terminus can bind integrin beta-4.
翻訳後修飾

Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
細胞内局在

Cytoplasm > cytoskeleton. Cell junction > hemidesmosome.
Target information above from: UniProt accession Q15149 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 5339 Human
- Entrez Gene: 18810 Mouse
- Entrez Gene: 64204 Rat
- Omim: 601282 Human
- SwissProt: Q15149 Human
- SwissProt: Q9QXS1 Mouse
- SwissProt: P30427 Rat
- Unigene: 434248 Human
- Unigene: 234912 Mouse
- Unigene: 1085 Rat
see all
別名
- EBS1 antibody
- EBSO antibody
- HD1 antibody
- Hemidesmosomal protein 1 antibody
- PCN antibody
- pleC antibody
- PLEC_HUMAN antibody
- PLEC1 antibody
- PLEC1b antibody
- Plectin 1 antibody
- Plectin 1 intermediate filament binding protein 500kDa antibody
- Plectin 6 antibody
- Plectin antibody
- Plectin-1 antibody
- PLTN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (ab32528)

Immunohistochemical staining of paraffin-embedded human skeletal muscle using ab32528 at a dilution of 1/250

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Plectin antibody [E398P] (ab32528)

All lanes : Anti-Plectin antibody [E398P] (ab32528) at 1/5000 dilution

Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : PLEC knockout U-2 OS cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 532 kDa

ab32528 was shown to react with PLEC in wild-type U-2 OS cells in Western blot with loss of signal observed in a PLEC knockout cell line. Wild-type U-2 OS and PLEC knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab32528 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plectin antibody [E398P] (ab32528)

Fluorescent immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab32528. Green-Plectin red-PI

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Plectin antibody [E398P] (ab32528)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab32528 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32528 は 35 報の論文で使用されています。

Del Bondio A et al. Restoring calcium homeostasis in Purkinje cells arrests neurodegeneration and neuroinflammation in the ARSACS mouse model. JCI Insight 8:N/A (2023). PubMed: 37159335
Plá V et al. Structural characterization of SLYM-a 4th meningeal membrane. Fluids Barriers CNS 20:93 (2023). PubMed: 38098084
Mouron S et al. Phosphoproteomic analysis of neoadjuvant breast cancer suggests that increased sensitivity to paclitaxel is driven by CDK4 and filamin A. Nat Commun 13:7529 (2022). PubMed: 36477027
Xu R et al. Plectin Downregulation Inhibits Migration and Suppresses Epithelial Mesenchymal Transformation of Hepatocellular Carcinoma Cells via ERK1/2 Signaling. Int J Mol Sci 24:N/A (2022). PubMed: 36613521
Buckup M et al. Plectin is a regulator of prostate cancer growth and metastasis. Oncogene 40:663-676 (2021). PubMed: 33219316

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-6 of 6 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (NHEK)

Permeabilization

Yes - Tween 0.1%

Specification

NHEK

Blocking step

BSA 1% + Goat serum 10% + Glycine 0.3mM as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C

Fixative

Formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 03 2023

Application

Western blot

Sample

Human Cell lysate - other (HeLa)

Gel Running Conditions

Reduced Denaturing

Loading amount

1.02e+006 cells

Specification

HeLa

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 07 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Epithelial cells)

Permeabilization

Yes - Triton

Specification

Epithelial cells

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

投稿 Sep 16 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (oral mucosa)

Permeabilization

Specification

oral mucosa

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

投稿 Jun 03 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Gallbladder)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)

Permeabilization

Specification

Gallbladder

Blocking step

Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 23 2018

Question

BATCH NUMBER 224205 ORDER NUMBER 20025832 DESCRIPTION OF THE PROBLEM The enduser doesn't see the protein after using the product on the coverslip with cells after fixation. SAMPLE Cells (human and mouse) PRIMARY ANTIBODY plectin antibody Dilution: (1:50), (1:150), (1:200) and (1:250) DETECTION METHOD CLSM POSITIVE AND NEGATIVE CONTROLS USED other antibodies against this protein ANTIBODY STORAGE CONDITIONS 4 degrees FIXATION OF SAMPLE 10'1%PFA or 15'100% methanol (-20 degrees) ANTIGEN RETRIEVAL -- PERMEABILIZATION STEP 5'0,5% triton-X-100 BLOCKING CONDITIONS 2% BSA SECONDARY ANTIBODY goat anti-rabbit tr 1:400 (dilution) HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I used two different fixations. ADDITIONAL NOTES I wonder if this product can be used for IF.

Abcam community

Verified customer

Asked on Jan 11 2007

Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Rabbit monoclonal [E398P] to Plectin (ab32528) is yet to be tested for use by immunocytochemistry and therefore we cannot guarantee it for this purpose. I have read your technical questionnaire and I have a few comments. Plectin is a cytoskeletal protein that has the potential to be extracted when using a permeabilization agent. My concern is that the triton treatment that you are performing is resulting in the loss of this protein or at least a degree of removal of the epitope from your cells. I would like to recommend the use of acetone as a fixative or the omission of triton in your existing protocol. If this does not lead to an improvement in the results that you have been obtaining please get back in touch with me and I will provide you with a free of charge replacement (provided that the antibody was purchased within the past 90 days).

Abcam Scientific Support

Answered on Jan 18 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (35)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Plectin antibody [E398P]

Western blot abreview for Anti-Plectin antibody [E398P]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Plectin antibody [E398P]

Immunohistochemistry (Frozen sections) abreview for Anti-Plectin antibody [E398P]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Plectin antibody [E398P]