Anti-PLD2 (phospho Y169) 抗体 (ab78907)
Key features and details
- Rabbit polyclonal to PLD2 (phospho Y169)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PLD2 (phospho Y169) antibody
PLD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PLD2 (phospho Y169) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human PLD2 (phospho Y169).
Database link: NP_002654 -
ポジティブ･コントロール
- IHC-P: human brain tissue WB: extracts from Jurkat cells treated with TNF (20 ng/ml, 30 min)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab78907 was purified by affinity chromatography using an epitope specific phosphopeptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78907の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 106 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 106 kDa. |
|
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
May have a role in signal-induced cytoskeletal regulation and/or endocytosis. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the phospholipase D family.
Contains 1 PH domain.
Contains 2 PLD phosphodiesterase domains.
Contains 1 PX (phox homology) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5338 Human
- Entrez Gene: 18806 Mouse
- Entrez Gene: 25097 Rat
- Omim: 602384 Human
- SwissProt: O14939 Human
- SwissProt: P97813 Mouse
- SwissProt: P70498 Rat
- Unigene: 104519 Human
see all -
別名
- Choline phosphatase 2 antibody
- EC 3.1.4.4 antibody
- hPLD 2 antibody
see all
画像
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Western blot - Anti-PLD2 (phospho Y169) antibody (ab78907)All lanes : Anti-PLD2 (phospho Y169) antibody (ab78907) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min)
Lane 2 : extracts from Jurkat cells, treated with TNF (20 ng/ml, 30 min) with immunizing peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 106 kDa
Observed band size: 94 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLD2 (phospho Y169) antibody (ab78907)Immunohistochemical analysis of PLD2 in paraffin embedded human brain tissue using ab78907, at 1/50 dilution, in the presence (right image) and absence (left image) of the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab78907 は 3 報の論文で使用されています。
- Wu H et al. Phospholipase D2 targeted by miR-5132-5p alleviates cerulein-induced acute pancreatitis via the Nrf2/NFκB pathway. Immun Inflamm Dis 11:e831 (2023). PubMed: 37249288
- Xuan Y et al. Inhibition of chaperone-mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer. Mol Med Rep 23:N/A (2021). PubMed: 33760140
- Chang YC et al. Aldolase A and Phospholipase D1 Synergistically Resist Alkylating Agents and Radiation in Lung Cancer. Front Oncol 11:811635 (2021). PubMed: 35127525