Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-PLCL1 抗体 [EPR11213] (ab157200)

Reviews (1) Submit a question References (1)
Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
  • Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
  • Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
  • Flow Cytometry (Intracellular) - Anti-PLCL1 antibody [EPR11213] (ab157200)
  • Anti-PLCL1 antibody [EPR11213] (ab157200)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11213] to PLCL1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PLCL1 antibody [EPR11213]
    PLCL1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11213] to PLCL1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment corresponding to Human PLCL1.

  • ポジティブ･コントロール

    • Jurkat cell lysate; fetal brain, fetal kidney and fetal lung cell lysates; Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20ºC.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11213

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab157200の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 123 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Predicted molecular weight: 123 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Involved in an inositol phospholipid-based intracellular signaling cascade. Shows no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. Component in the phospho-dependent endocytosis process of GABA A receptor (By similarity). Regulates the turnover of receptors and thus contributes to the maintenance of GABA-mediated synaptic inhibition. Its aberrant expression could contribute to the genesis and progression of lung carcinoma. Acts as a inhibitor of PPP1C.

  • 組織特異性

    Expressed in a variety of fetal and adult organs including brain, lung and kidney. Its expression was greatly reduced in small and non-small cell lung carcinoma. Isoform 1 is predominantly expressed in brain.

  • 配列類似性

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.

  • 翻訳後修飾

    Phosphorylated by the catalytic subunit of PKA. Phosphorylation of Thr-93 resulted in dissociation of PPP1C from PRIP1.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Inactive phospholipase C-like protein 1 antibody
      • Inactive protein 1 antibody
      • Phospholipase C deleted in lung carcinoma antibody
      • Phospholipase C epsilon antibody
      • Phospholipase C like 1 antibody
      • Phospholipase C related but catalytically inactive protein antibody
      • Phospholipase C related, but catalytically inactive protein antibody
      • Phospholipase C-deleted in lung carcinoma antibody
      • Phospholipase C-related but catalytically inactive protein antibody
      • Phospholipase C-related catalytically antibody
      • PLC-L1 antibody
      • PLCE antibody
      • PLCL antibody
      • PLCL1 antibody
      • PLCL1_HUMAN antibody
      • PLDL1 antibody
      • PRIP antibody
      see all

    画像

    • Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
      Immunoprecipitation - Anti-PLCL1 antibody [EPR11213] (ab157200)
      Western blot analysis of Immunoprecipitation pellet from Human fetal brain lysate showing PLCL1, using ab157200 at 1/10 dilution for IP.
    • Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
      Western blot - Anti-PLCL1 antibody [EPR11213] (ab157200)
      All lanes : Anti-PLCL1 antibody [EPR11213] (ab157200) at 1/10000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Human fetal kidney tissue lysate
      Lane 3 : Human fetal lung tissue lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 123 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
      Immunocytochemistry/ Immunofluorescence - Anti-PLCL1 antibody [EPR11213] (ab157200)
      Immunofluorescence analysis of Jurkat cells, labeling PLCL1 using ab157200 at a 1/100 dilution.
    • Flow Cytometry (Intracellular) - Anti-PLCL1 antibody [EPR11213] (ab157200)
      Flow Cytometry (Intracellular) - Anti-PLCL1 antibody [EPR11213] (ab157200)

      Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling PLCL1 (red), using ab157200 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

       

    • Anti-PLCL1 antibody [EPR11213] (ab157200)
      Anti-PLCL1 antibody [EPR11213] (ab157200)

    参考文献 (1)

    ab157200 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157200 は 1 報の論文で使用されています。

    • Xiong Z  et al. Tumor Cell "Slimming" Regulates Tumor Progression through PLCL1/UCP1-Mediated Lipid Browning. Adv Sci (Weinh) 6:1801862 (2019). PubMed: 31131187

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Frozen sections) abreview for Anti-PLCL1 antibody [EPR11213]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.01% triton
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com