Anti-PLCG 2 (phospho Y759) 抗体 [EP1406Y] (ab51020)
![Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)](https://www.abcam.co.jp/ps/products/51/ab51020/Images/ab51020-62852-anti-plcg-2-phospho-y759-antibody-ep1406y-western-blot.jpg "Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759)
- Suitable for: ICC/IF, WB
- Reacts with: Human
製品の概要
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製品名
Anti-PLCG 2 (phospho Y759) antibody [EP1406Y]
PLCG 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1406Y] to PLCG 2 (phospho Y759) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Raji cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1406Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51020の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
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| WB | (1) |
1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa).
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| 特記事項 |
|---|
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ICC/IF
1/50 - 1/100. |
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WB
1/50000 - 1/100000. Detects a band of approximately 150 kDa (predicted molecular weight: 148 kDa). |
ターゲット情報
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機能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
配列類似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻訳後修飾
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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参照データベース
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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別名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
画像
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Western blot - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)All lanes : Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020) at 1/100000 dilution
Lane 1 : Raji cell lysate, untreated
Lane 2 : Raji cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 148 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)](https://www.abcam.co.jp/ps/products/51/ab51020/Images/ab51020-2-anti-plcg-2-antibody-ep1406y-immunocytochemistry-raji-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y759) antibody [EP1406Y] (ab51020)Immunocytochemistry analysis of Raji (human Burkitt's lymphoma B lymphocyte) labeling PLCG 2 with purified ab51020 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.12 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab51020 は 2 報の論文で使用されています。
- Li L et al. Asebogenin suppresses thrombus formation via inhibition of Syk phosphorylation. Br J Pharmacol 180:287-307 (2023). PubMed: 36166754
- Mohammad DK et al. Dual phosphorylation of Btk by Akt/protein kinase b provides docking for 14-3-3?, regulates shuttling, and attenuates both tonic and induced signaling in B cells. Mol Cell Biol 33:3214-26 (2013). PubMed: 23754751