Anti-PLCG 2 (phospho Y753) 抗体 (ab75659)
Key features and details
- Rabbit polyclonal to PLCG 2 (phospho Y753)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PLCG 2 (phospho Y753) antibody
PLCG 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PLCG 2 (phospho Y753) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PLCG 2 (phospho Y753).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab75659 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75659の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/200.
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| WB |
1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
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| 特記事項 |
|---|
|
ICC/IF
1/100 - 1/200. |
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WB
1/500 - 1/1000. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa). |
ターゲット情報
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機能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. -
配列類似性
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
翻訳後修飾
Phosphorylated on tyrosine residues; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Increases phospholipase activity. - Information by UniProt
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参照データベース
- Entrez Gene: 5336 Human
- Omim: 600220 Human
- SwissProt: P16885 Human
- Unigene: 413111 Human
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別名
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody
- 1-phosphatidylinositol-4 antibody
- 5-bisphosphate phosphodiesterase gamma-2 antibody
see all
画像
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Western blot - Anti-PLCG 2 (phospho Y753) antibody (ab75659)All lanes : Anti-PLCG 2 (phospho Y753) antibody (ab75659) at 1/500 dilution
Lane 1 : A431 cell extracts, untreated
Lane 2 : A431 cell extracts treated with EGF (200ng/ml, 5min)
Predicted band size: 148 kDa
Observed band size: 148 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-PLCG 2 (phospho Y753) antibody (ab75659)ab75659 at 1/100 dilution staining PLCG 2 (phospho Y753) in human Hela cells by ICC/IF.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab75659 は 1 報の論文で使用されています。
- Sadeghi L et al. Differential B-Cell Receptor Signaling Requirement for Adhesion of Mantle Cell Lymphoma Cells to Stromal Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32370190