Anti-Plastin L 抗体 [EPR4277] (ab109124)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4277] to Plastin L
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-Plastin L antibody [EPR4277]
Plastin L 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4277] to Plastin L
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- THP1, K562 and Jurkat whole cell lysate (ab7899); Human tonsil tissue WB: Rat spleen, Mouse spleen IHC-P: Mouse spleen, mouse colon, rat spleen
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4277
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Plastin L antibody [EPR4277] - BSA and Azide free (ab247772)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Jurkat cytoplasmic extract lysate (ab14842)
Recombinant Protein
- Recombinant Human Plastin L protein (Tagged) (ab235562)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109124の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C allow to cool for 10-20 min).

追加情報

Is unsuitable for Flow Cyt or IP.

機能

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
組織特異性

Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T lymphocytes, neutrophils, monocytes, B lymphocytes, and myeloid cells.
配列類似性

Contains 2 actin-binding domains.
Contains 4 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
翻訳後修飾

Phosphorylated on a serine residue in response to costimulation through TCR/CD3 and CD2 or CD28. Serine phosphorylation promotes association with the actin cytoskeleton and targeting to peripheral cell projections.
The N-terminus is blocked.
細胞内局在

Cytoplasm > cytoskeleton. Cell junction. Cell projection. Cell projection > ruffle membrane. Relocalizes to the immunological synapse between peripheral blood T lymphocytes and antibody-presenting cells in response to costimulation through TCR/CD3 and CD2 or CD28. Associated with the actin cytoskeleton at membrane ruffles (By similarity). Relocalizes to actin-rich cell projections upon serine phosphorylation.
Target information above from: UniProt accession P13796 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 3936 Human
- Entrez Gene: 18826 Mouse
- Entrez Gene: 306071 Rat
- Omim: 153430 Human
- SwissProt: P13796 Human
- SwissProt: Q61233 Mouse
- Unigene: 381099 Human
- Unigene: 710563 Human
- Unigene: 153911 Mouse
see all
別名
- CP64 antibody
- L plastin antibody
- L-plastin antibody
- Larval cuticle protein 1 antibody
- LC64P antibody
- LCP-1 antibody
- LCP1 antibody
- LPL antibody
- Lplastin antibody
- Lymphocyte cytosolic protein 1 antibody
- Plastin 2 antibody
- Plastin-2 antibody
- PLS2 antibody
- PLSL_HUMAN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Rat testis labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control: Negligible staining on Rat testis.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Mouse liver labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control: Negligible staining on mouse liver.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Mouse testis labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control: Negligible staining on mouse testis.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression control: no staining on the cardiac muscle in mouse heart, but staining the stroma immune cells.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Rat spleen labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on the rat spleen.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Mouse spleen labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on the mouse spleen.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

Immunohistochemical analysis of paraffin-embedded Mouse colon labelling Plastin L with ab109124 at 1/400 dilution (0.35 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on the immune cells in mouse colon.

The section was incubated with ab109124 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)

All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

Lane 1 : Low expression control: Rat brain tissue lysate
Lane 2 : Low expression control: Rat liver tissue lysate
Lane 3 : Low expression control: Rat testis tissue lysate
Lane 4 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 10 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Low expression control: Rat brain, rat liver, rat testis.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)

All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

Lane 1 : Low expression control: Mouse brain tissue lysate
Lane 2 : Low expression control: Mouse liver tissue lysate
Lane 3 : Low expression control: Mouse testis tissue lysate
Lane 4 : Mouse spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 10 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Low expression control: mouse brain, mouse liver, mouse testis.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
Western blot - Anti-Plastin L antibody [EPR4277] (ab109124)

All lanes : Anti-Plastin L antibody [EPR4277] (ab109124) at 1/1000 dilution

Lane 1 : THP1 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : Jurkat cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plastin L antibody [EPR4277] (ab109124)

ab109124, at 1/100 dilution staining Plastin L in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Plastin L antibody [EPR4277] (ab109124)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab109124 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109124 は 3 報の論文で使用されています。

Li X et al. Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss. Sci Adv 6:N/A (2020). PubMed: 33208358
Wang LL et al. Platelet-derived growth factor subunit B is required for tendon-bone healing using bone marrow-derived mesenchymal stem cells after rotator cuff repair in rats. J Cell Biochem 119:8897-8908 (2018). PubMed: 30105826
Lapek JD et al. Defining Host Responses during Systemic Bacterial Infection through Construction of a Murine Organ Proteome Atlas. Cell Syst 6:579-592.e4 (2018). PubMed: 29778837

レビューと Q&A

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レビューを送る質問を送る

Application

Western blot

Sample

Dog Cell lysate - whole cell (canine mammary gland tumor)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

20 µg

Specification

canine mammary gland tumor

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jun 23 2021

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (3)

レビューと Q&A

Western blot abreview for Anti-Plastin L antibody [EPR4277]