Anti-Plasminogen 抗体 [EPSISR18(2)] (ab174285)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR18(2)] to Plasminogen
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Plasminogen antibody [EPSISR18(2)]
Plasminogen 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPSISR18(2)] to Plasminogen -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Plasminogen aa 650-750 (Cysteine residue). The exact sequence is proprietary.
Database link: P00747 -
ポジティブ・コントロール
- Natural human Plasminogen protein (ab77879) can be used as a positive control in WB. Human plasma lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPSISR18(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174285の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 90 kDa.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 90 kDa. |
ターゲット情報
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機能
Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. -
組織特異性
Present in plasma and many other extracellular fluids. It is synthesized in the liver. -
関連疾患
Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. -
配列類似性
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 5 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
ドメイン
Kringle domains mediate interaction with CSPG4. -
翻訳後修飾
N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide. -
細胞内局在
Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 5340 Human
- Omim: 173350 Human
- SwissProt: P00747 Human
- Unigene: 143436 Human
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製品の状態
Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B -
別名
- Plasmin antibody
- Plasmin heavy chain A antibody
- Plasmin light chain B antibody
see all
画像
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Anti-Plasminogen antibody [EPSISR18(2)] (ab174285) at 1/1000 dilution (purified) + Human plasma lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 90 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-Plasminogen antibody [EPSISR18(2)] (ab174285) at 1/1000 dilution + Human plasma lysate at 10 µg
Secondary
Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 90 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab174285 は論文での使用が確認できていません。