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リコンビナントRabMAb

Anti-Plasminogen 抗体 [EPR22406-159] - BSA and Azide free (ab245832)

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Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free (ab245832)
  • Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free (ab245832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22406-159] to Plasminogen - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free
    Plasminogen 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22406-159] to Plasminogen - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: IHC-Fr or IHC-P

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IP: Mouse liver lysate.

  • 特記事項

    ab245832 is the carrier-free version of ab245247.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245832の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 91 kDa).
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-Fr or IHC-P.

ターゲット情報

  • 機能

    Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.
    Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.

  • 組織特異性

    Present in plasma and many other extracellular fluids. It is synthesized in the liver.

  • 関連疾患

    Defects in PLG are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.
    Defects in PLG are the cause of plasminogen deficiency (PLGD) [MIM:217090]. PLGD is characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa.

  • 配列類似性

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 5 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.

  • ドメイン

    Kringle domains mediate interaction with CSPG4.

  • 翻訳後修飾

    N-linked glycan contains N-acetyllactosamine and sialic acid. O-linked glycans consist of Gal-GalNAc disaccharide modified with up to 2 sialic acid residues (microheterogeneity).
    In the presence of the inhibitor, the activation involves only cleavage after Arg-580, yielding two chains held together by two disulfide bonds. In the absence of the inhibitor, the activation involves additionally the removal of the activation peptide.

  • 細胞内局在

    Secreted. Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      Cleaved into the following 5 chains: 1.Plasmin heavy chain A2.Activation peptide3.Angiostatin4.Plasmin heavy chain A, short form5. Plasmin light chain B

    • 別名

      • Plasmin antibody
      • Plasmin heavy chain A antibody
      • Plasmin light chain B antibody
      • Plasminogen antibody
      • PLG antibody
      • PLMN_HUMAN antibody
      see all

    画像

    • Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free (ab245832)
      Immunoprecipitation - Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free (ab245832)

      Plasminogen was immunoprecipitated from 0.35 mg of mouse liver lysate with ab245247 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245247 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: Mouse liver lysate 10 µg (Input). 

      Lane 2: ab245247 IP in mouse liver lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245247 in mouse liver lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245247).

    • Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free (ab245832)
      Anti-Plasminogen antibody [EPR22406-159] - BSA and Azide free (ab245832)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245832 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245832 は論文での使用が確認できていません。

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