AB124938

Anti-Plasma Kallikrein 1B 抗体 [EPR5707]

Anti-Plasma Kallikrein 1B antibody [EPR5707]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Plasma Kallikrein 1B antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

ab124938 at 1/50 dilution staining Plasma Kallikrein 1B in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

All lanes:

Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (ab124938) at 1/1000 dilution

Lane 1:

Human breast cancer lysate at 10 µg

Lane 2:

Human plasma lysate at 10 µg

Lane 3:

Human ovary lysate at 10 µg

Lane 4:

Fetal liver tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 71 kDa

Observed band size: 80 kDa

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OI-RD Scanning - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

OI-RD Scanning

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OI-RD Scanning - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5707

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.

Biological function summary

Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.

Pathways

Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.

Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

See full target information KLKB1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40960171

2025

A novel mouse model for -related muscular dystrophy with analysis of molecular pathogenesis and clinical phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Tan,Yidan Liu,Huaxia Luo,Qiang Shen,Xingbo Long,Luzheng Xu,Jieyu Liu,Nanbert A Zhong,Hong Zhang,Hui Xiong

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Plasma Kallikrein 1B 抗体 [EPR5707]

Anti-Plasma Kallikrein 1B antibody [EPR5707]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

Western blot - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

OI-RD Scanning - Anti-Plasma Kallikrein 1B antibody [EPR5707] (AB124938)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Abcam product promise