AB1006

Anti-Plasma Kallikrein 1B 抗体 [13G11]

Name: Anti-Plasma Kallikrein 1B 抗体 [13G11] (ab1006) | Abcam
Brand: Abcam Limited
SKU: AB1006
Price: 65000 JPY
Availability: InStock
Rating: 4 (3 reviews)

Anti-Plasma Kallikrein 1B antibody [13G11]

(3 Reviews)

(12 Publications)

Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006) is a mouse monoclonal antibody detecting Plasma Kallikrein 1B in IHC-P. Suitable for Human.

- Over 10 publications
- Trusted since 2002

別名を表示する

KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [13G11] (AB1006)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [13G11] (AB1006)

ab1006 (4μg/ml) staining plasma kallikrein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining of a subpopulation of hepatocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

This image was generated using the ascites version of the product.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

13G11

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

IHC-P

applications

免疫原

Native Full Length Protein corresponding to Human KLKB1.

P03952

エピトープ

The specific epitope, found on the prekallikrein / kallikrein heavy chain is involved in the interaction between prekallikrein and factor XIIa.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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製品の詳細

What is this antibody validated in?
Anti-Plasma Kallikrein 1B antibody [13G11] (ab1006) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-Plasma Kallikrein 1B [13G11] (ab1006) was first used in a scientific publication in 2002 and has been cited over 10 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

Purified from TCS

バッファー組成

pH: 7.4

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.

Biological function summary

Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.

Pathways

Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.

Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

See full target information KLKB1

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:155 PubMed38872149

2024

Delayed plasma kallikrein inhibition fosters post-stroke recovery by reducing thrombo-inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Steffen Haupeltshofer,Stine Mencl,Rebecca D Szepanowski,Christina Hansmann,Ana I Casas,Hanna Abberger,Wiebke Hansen,Alina Blusch,Cornelius Deuschl,Michael Forsting,Dirk M Hermann,Friederike Langhauser,Christoph Kleinschnitz

Neuroscience 413:294-307 PubMed31181367

2019

A Short Isoform of Coagulation Factor XII mRNA Is Expressed by Neurons in the Human Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Zamolodchikov,Yu Bai,Yajun Tang,John R McWhirter,Lynn E Macdonald,Nicole Alessandri-Haber

International journal of molecular sciences 20: PubMed30791599

2019

Integrated Proteomics and Metabolomic Analyses of Plasma Injury Biomarkers in a Serious Brain Trauma Model in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Song,Ying Zhu,Peng Zhang,Minzhu Zhao,Dezhang Zhao,Shijia Ding,Shisheng Zhu,Jianbo Li

Current urology 9:192-201 PubMed28413380

2017

Expression of Epidermal Growth Factor Receptor and Transforming Growth Factor Alpha in Cancer Bladder: Schistosomal and Non-Schistosomal.

Applications

Unspecified application

Species

Unspecified reactive species

Afkar A Badawy,Ali El-Hindawi,Olfat Hammam,Mona Moussa,Noha S Helal,Amira Kamel

Annals of neurology 77:784-803 PubMed25628066

2015

Blocking of plasma kallikrein ameliorates stroke by reducing thromboinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Göb,Stephan Reymann,Friederike Langhauser,Michael K Schuhmann,Peter Kraft,Ina Thielmann,Kerstin Göbel,Marc Brede,György Homola,László Solymosi,Guido Stoll,Christian Geis,Sven G Meuth,Bernhard Nieswandt,Christoph Kleinschnitz

Nature medicine 17:206-10 PubMed21258336

2011

Hyperglycemia-induced cerebral hematoma expansion is mediated by plasma kallikrein.

Applications

FuncS

Species

Rat

Jia Liu,Ben-Bo Gao,Allen C Clermont,Price Blair,Tamie J Chilcote,Sukanto Sinha,Robert Flaumenhaft,Edward P Feener

American journal of physiology. Cell physiology 296:C514-24 PubMed19109528

2008

Autonomic nervous system regulates secretion of anti-inflammatory prohormone SMR1 from rat salivary glands.

Applications

Species

Rat

K E Morris,C D St Laurent,R S Hoeve,P Forsythe,M R Suresh,R D Mathison,A D Befus

Nature medicine 13:181-8 PubMed17259996

2007

Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ben-Bo Gao,Allen Clermont,Susan Rook,Stephanie J Fonda,Vivek J Srinivasan,Maciej Wojtkowski,James G Fujimoto,Robert L Avery,Paul G Arrigg,Sven-Erik Bursell,Lloyd Paul Aiello,Edward P Feener

Immunopharmacology 33:374-6 PubMed8856190

1996

Does resistance to endotoxin in primates correlate with levels of plasma prekallikrein?

Applications

Unspecified application

Species

Unspecified reactive species

D Veloso

Thrombosis and haemostasis 68:526-33 PubMed1455399

1992

Structure, kinetics, and function of human and rhesus plasma prekallikreins are similar.

Applications

Unspecified application

Species

Unspecified reactive species

D Veloso,J I Smith,S Denny,T M Cosgriff

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Plasma Kallikrein 1B 抗体 [13G11]

Anti-Plasma Kallikrein 1B antibody [13G11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plasma Kallikrein 1B antibody [13G11] (AB1006)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (12)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise