AB16695

Anti-Placental alkaline phosphatase (PLAP) 抗体 [SP15]

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(13 Publications)

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695) is a rabbit monoclonal antibody detecting Placental alkaline phosphatase (PLAP) in Flow Cytometry (Intra), IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2005

別名を表示する

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

2 Images

Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Intracellular flow cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Placental alkaline phosphatase (PLAP) with purified ab16695 at 1/200 dilution (0.6μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

This image was generated from the hybridoma version.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Human seminoma stained with ab16695.

This image was generated from the hybridoma version.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP15

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

エピトープ

Not determined

特異性

This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (ab16695) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Collaboration
Anti-Placental alkaline phosphatase (PLAP) [SP15] (ab16695) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trusted by the scientific community
Anti-Placental alkaline phosphatase (PLAP) [SP15] (ab16695) was first used in a scientific publication in 2005 and has been cited over 10 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [SP15] also available for your convenience: ab16695, Carrier free - ab218848, ab238800, Alexa Fluor® 488 - ab270195

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.

Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPP

style

left-column

文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e12413 PubMed38353485

2024

Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk.

Applications

Unspecified application

Species

Unspecified reactive species

Miira M Klemetti,Ante B V Pettersson,Aafaque Ahmad Khan,Leonardo Ermini,Tyler R Porter,Michael L Litvack,Sruthi Alahari,Stacy Zamudio,Nicholas P Illsley,Hannes Röst,Martin Post,Isabella Caniggia

Stem cell research & therapy 12:64 PubMed33461605

2021

The miR-204-5p/FOXC1/GDF7 axis regulates the osteogenic differentiation of human adipose-derived stem cells via the AKT and p38 signalling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

You Zhou,Siyu Liu,Wei Wang,Qiang Sun,Mengzhu Lv,Shude Yang,Shuang Tong,Shu Guo

The Journal of biological chemistry 295:16393-16410 PubMed32967966

2020

Calnexin mediates the maturation of GPI-anchors through ER retention.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Guo,Yi-Shi Liu,Xiao-Dong Gao,Taroh Kinoshita,Morihisa Fujita

Genesis (New York, N.Y. : 2000) 58:e23359 PubMed32191380

2020

Generation of an Fsp1 (fibroblast-specific protein 1)-Flpo transgenic mouse strain.

Applications

Unspecified application

Species

Unspecified reactive species

Victoire Cardot-Ruffino,Véronique Chauvet,Cassandre Caligaris,Adrien Bertrand-Chapel,Nicolas Chuvin,Roxane M Pommier,Ulrich Valcourt,David Vincent,Sylvie Martel,Sophie Aires,Bastien Kaniewski,Pierre Dubus,Philippe Cassier,Stéphanie Sentis,Laurent Bartholin

Cells, tissues, organs 207:97-113 PubMed31655811

2019

Investigating the Osteoinductive Potential of a Decellularized Xenograft Bone Substitute.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel N Bracey,Alexander H Jinnah,Jeffrey S Willey,Thorsten M Seyler,Ian D Hutchinson,Patrick W Whitlock,Thomas L Smith,Kerry A Danelson,Cynthia L Emory,Bethany A Kerr

eLife 8: PubMed30714906

2019

Transsynaptic interactions between IgSF proteins DIP-α and Dpr10 are required for motor neuron targeting specificity.

Applications

Unspecified application

Species

Unspecified reactive species

James Ashley,Violet Sorrentino,Meike Lobb-Rabe,Sonal Nagarkar-Jaiswal,Liming Tan,Shuwa Xu,Qi Xiao,Kai Zinn,Robert A Carrillo

Molecular carcinogenesis 58:621-626 PubMed30582228

2018

cd44 deletion suppresses atypia in the precancerous mouse testis.

Applications

Unspecified application

Species

Unspecified reactive species

Huaibiao Li,Shalmali Shukla,Lucien Frappart,Peter Herrlich,Aspasia Ploubidou

Retrovirology 10:4 PubMed23305456

2013

Virion stiffness regulates immature HIV-1 entry.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Bo Pang,Liron Hevroni,Nitzan Kol,Debra M Eckert,Marianna Tsvitov,Michael S Kay,Itay Rousso

The Journal of biological chemistry 287:32525-34 PubMed22851169

2012

Nicotinic acid adenine dinucleotide phosphate (NAADP) degradation by alkaline phosphatase.

Applications

Species

Unspecified reactive species

Frederike Schmid,Ralf Fliegert,Tim Westphal,Andreas Bauche,Andreas H Guse

Journal of andrology 33:505-13 PubMed21868749

2011

Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice.

Applications

IHC-P

Species

Mouse

Camelia M Saffarini,Nicholas E Heger,Hideki Yamasaki,Tao Liu,Susan J Hall,Kim Boekelheide

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Placental alkaline phosphatase (PLAP) 抗体 [SP15]

Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]

Flow Cytometry (Intracellular) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] (AB16695)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

エピトープ

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (13)

Abcam product promise