AB133602

Anti-ALPP+ALPG+ALPI 抗体 [EPR6141]

Name: Anti-Placental alkaline phosphatase (PLAP) 抗体 [EPR6141] (ab133602) | Abcam
Brand: Abcam Limited
SKU: AB133602
Price: 38000 JPY
Availability: InStock
Rating: 4 (2 reviews)

Anti-ALPP+ALPG+ALPI antibody [EPR6141]

20ul selling size
RabMAb
Recombinant
詳細を見る

(2 Reviews)

(29 Publications)

Rabbit Recombinant Monoclonal PPB1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 29 publications.

別名を表示する

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1, Intestinal-type alkaline phosphatase, IAP, Intestinal alkaline phosphatase, ALPI, ALPPL, ALPPL2, ALPG, ALP-1, Alkaline phosphatase Nagao isozyme, Germ cell alkaline phosphatase, Placental alkaline phosphatase-like, GCAP, PLAP-like

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemical analysis of paraffin-embedded Human dysgerminoma tissue labelling Placental alkaline phosphatase (PLAP) with ab133602 at 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing positive staining in Seminoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing positive staining in Normal placenta tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

All lanes:

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (ab133602) at 1/10000 dilution

Lane 1:

Human placenta tissue lysate

Lane 2:

Human seminoma tissue lysate

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 70 kDa

false

Unknown

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Lanes 1-4 : Merged signal (red and green). Green - ab133602 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab133602 Anti-Placental alkaline phosphatase (PLAP) antibody [EPR6141] was shown to specifically react with Placental alkaline phosphatase (PLAP) in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261763 (knockout cell lysate ab256836) was used. Wild-type and Placental alkaline phosphatase (PLAP) knockout samples were subjected to SDS-PAGE. ab133602 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (ab133602) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ALPP knockout HeLa cell lysate at 20 µg

Lane 3:

Human placenta tissue lysate at 20 µg

Lane 4:

Human heart tissue lysate at 20 µg

Predicted band size: 58 kDa

Observed band size: 70 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

ab133602 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

OI-RD Scanning - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR6141

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.

Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPP

追加のターゲット

ALPG,ALPI

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic translation 50:144-157 PubMed40171108

2025

Moderate static magnetic field regulates iron metabolism and salvage bone loss caused by iron accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxiao Zhen,Shenghang Wang,Jiancheng Yang,Gejing Zhang,Chao Cai,Jianping Wang,Aifei Wang,Youjia Xu,Yanwen Fang,Min Wei,Dachuan Yin,Xinle Luo,Ming Gong,Hao Zhang,Peng Shang

Frontiers in cell and developmental biology 13:1528714 PubMed39996028

2025

The regulatory role of placental extracellular vesicle on trophoblast and endothelial cell functions.

Applications

Unspecified application

Species

Unspecified reactive species

Kunfeng Bai,Xintong Li,Yanjie Guo,Ye Shang,Leqian Lin,Philip C N Chiu,Cheuk-Lun Lee

Placenta 160:118-125 PubMed39793469

2025

Placental alkaline phosphatase (PLAP): Is it exclusively placental?

Applications

Unspecified application

Species

Unspecified reactive species

Awanit Kumar,Sourabh Sharma,Maged M Costantine,Kara Rood,Rheanna Urrabaz-Garza,Jeena Jacob,Lauren S Richardson,Ananth Kumar Kammala,Ramkumar Menon

Journal of extracellular biology 3:e135 PubMed38938672

2024

Differential response of placental cells to high D-glucose and its impact on extracellular vesicle biogenesis and trafficking via small GTPase Ras-related protein RAB-7A.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Palma,Andrew Lai,Katherin Scholz-Romero,Haarika Chittoory,Benjamin Van Haeringen,Flavio Carrion,Aase Handberg,Martha Lappas,Sunil R Lakhani,Amy E McCart Reed,H David McIntyre,Soumyalekshmi Nair,Carlos Salomon

Advanced healthcare materials 13:e2302879 PubMed37927129

2023

3D Printed Photothermal Scaffold Sandwiching Bacteria Inside and Outside Improves The Infected Microenvironment and Repairs Bone Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Youzi Zhao,Honglei Kang,Yuhao Xia,Lingshun Sun,Feng Li,Honglian Dai

Journal of translational medicine 21:496 PubMed37488572

2023

Small extracellular vesicles-transported lncRNA TDRKH-AS1 derived from AOPPs-treated trophoblasts initiates endothelial cells pyroptosis through PDIA4/DDIT4 axis in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Jiexing He,Haihua Liu,Qiuyu Huang,Shuoshi Wang,Ailan Yin,Shuying Chen,Xinyang Shen,Yanxuan Xiao,Haoyue Hu,Jiayi Jiang,Wenqian Chen,Song Wang,Zhenqin Huang,Jiaqi Li,You Peng,Xiaocong Wang,Xinping Yang,Zhijian Wang,Mei Zhong

International journal of nanomedicine 18:641-657 PubMed36789391

2023

Gestational Diabetes Mellitus Impedes Fetal Lung Development Through Exosome-Dependent Crosstalk Between Trophoblasts and Lung Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Pengzheng Chen,Mengqi Gu,Shuting Wan,Xiaotong Jiang,Fengyuan Zhang,Yuchen Li,Qian Zhou,Yuan Lu,Lei Li,Xietong Wang

Open life sciences 17:1591-1599 PubMed36518887

2022

PHF8 promotes osteogenic differentiation of BMSCs in old rat with osteoporosis by regulating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Pan,Kai Huang,Hongbin Dai,Chunhe Sha

Cells 11: PubMed36359855

2022

Chemerin-Induced Down-Regulation of Placenta-Derived Exosomal miR-140-3p and miR-574-3p Promotes Umbilical Vein Endothelial Cells Proliferation, Migration, and Tube Formation in Gestational Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Zhang,Qi Wu,Shuqi Zhu,Yibo Tang,Yanmin Chen,Danqing Chen,Zhaoxia Liang

Cell reports 39:110962 PubMed35705034

2022

Genetic encoding of an esophageal motor circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana C Coverdell,Ruei-Jen Abraham-Fan,Chen Wu,Stephen B G Abbott,John N Campbell

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ALPP+ALPG+ALPI 抗体 [EPR6141]

Anti-ALPP+ALPG+ALPI antibody [EPR6141]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Western blot - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

OI-RD Scanning - Anti-ALPP+ALPG+ALPI antibody [EPR6141] (AB133602)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (29)

Abcam product promise