Anti-Placental alkaline phosphatase (PLAP) 抗体 [8B6] (ab33)
Key features and details
- Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]
Placental alkaline phosphatase (PLAP) 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8B6] to Placental alkaline phosphatase (PLAP) -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Placental alkaline phosphatase (PLAP). Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that have had their surface expression of PLAP boosted in culture.
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ポジティブ・コントロール
- WB: Human placenta tissue lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: 0.02% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
8B6 -
ミエローマ
P3-NS1/1-Ag4-1 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa).
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特記事項 |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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配列類似性
Belongs to the alkaline phosphatase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 250 Human
- Omim: 171800 Human
- SwissProt: P05187 Human
- Unigene: 284255 Human
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別名
- Alkaline phosphatase antibody
- Alkaline phosphatase placental antibody
- Alkaline phosphatase placental type antibody
see all
画像
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All lanes : Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) at 5 µg/ml
Lane 1 : Human placenta tissue lysate
Lane 2 : Human kidney tissue lysate (negative control)
Lane 3 : Human liver tissue lysate (negative control)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Predicted band size: 58 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesBlocking buffer: 3% milk.
Gel Type: MOPS.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab33 は 6 報の論文で使用されています。
- Hu Y et al. High-Mobility Group Box 1 From Hypoxic Trophoblasts Promotes Endothelial Microparticle Production and Thrombophilia in Preeclampsia. Arterioscler Thromb Vasc Biol 38:1381-1391 (2018). PubMed: 29650693
- Müller WEG et al. Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP. J Cell Sci 130:2747-2756 (2017). PubMed: 28687622
- Sharp TM et al. Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal. PLoS One 5:e13130 (2010). ICC/IF ; Human . PubMed: 20976190
- Zhang L et al. Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo. J Gene Med 7:354-65 (2005). ELISA ; Human . PubMed: 15619290
- Durbin H et al. Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments. Int J Cancer Suppl 2:59-66 (1988). PubMed: 3162446
- Durbin H et al. Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations. Int J Cancer Suppl 2:50-8 (1988). PubMed: 2450848