AB33

Anti-Placental alkaline phosphatase (PLAP) 抗体 [8B6]

Name: Anti-Placental alkaline phosphatase (PLAP) 抗体 [8B6] (ab33) | Abcam
Brand: Abcam Limited
SKU: AB33
Price: 56000 JPY
Availability: InStock

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]

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(6 Publications)

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) is a mouse monoclonal antibody detecting Placental alkaline phosphatase (PLAP) in Western Blot. Suitable for Human.

- Trusted since 1998

別名を表示する

PLAP, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1

1 Images

Lab

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (AB33)

Blocking buffer : 3% milk.

Gel Type : MOPS.

All lanes:

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) at 5 µg/mL

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

Human kidney tissue lysate (negative control) at 10 µg

Lane 3:

Human liver tissue lysate (negative control) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 65 kDa

false

Exposure time: 8min

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

8B6

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "<p></p>" } } }

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製品の詳細

What is this antibody validated in?
Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (ab33) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of Placental alkaline phosphatase (PLAP)?
Anti-Placental alkaline phosphatase (PLAP) [8B6] (ab33) specifically detects a band for Placental alkaline phosphatase (PLAP) (UniProt: P05187) at a molecular weight of 58kDa.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Placental alkaline phosphatase (PLAP) also known by names like alkaline phosphatase/PLAP protein or ALPP functions as an enzyme that removes phosphate groups from molecules. It typically appears in tissues such as the placenta and is known to be active during pregnancy. PLAP has a molecular mass of approximately 66 kDa. Normally found expressed in the placenta this enzyme shares similarities with other alkaline phosphatases but is distinct due to its specific expression and function during gestation.

Biological function summary

PLAP shows importance in bone mineralization and fetal development. It participates in the hydrolysis of phosphate esters influencing nutrient availability and metabolism. PLAP is not a part of major protein complexes; however its enzymatic activity directly affects local phosphate concentrations which are critical for processes like bone formation and tissue regeneration during pregnancy.

Pathways

PLAP expression and activity intersect significantly with phosphate metabolism and fetal development pathways. The alkaline phosphatase family plays central roles in these pathways and PLAP shares functions with other isoenzymes in the family such as the tissue nonspecific alkaline phosphatase. These enzymes regulate phosphate turnover and contribute to essential developmental processes.

PLAP connects primarily to certain types of cancers and gestational disorders. Elevated levels of PLAP link to testicular germ cell tumors and ovarian cancer where it serves as a biomarker. In gestational trophoblastic disease PLAP expression provides valuable diagnostic information. Interactions with proteins like hCG (human chorionic gonadotropin) also offer insights into potential disease mechanisms especially in reproductive health challenges.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Alkaline phosphatase that can hydrolyze various phosphate compounds.

See full target information ALPP

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Arteriosclerosis, thrombosis, and vascular biology 38:1381-1391 PubMed29650693

2018

High-Mobility Group Box 1 From Hypoxic Trophoblasts Promotes Endothelial Microparticle Production and Thrombophilia in Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Yae Hu,Ruhong Yan,Ce Zhang,Zhichao Zhou,Meng Liu,Can Wang,Hong Zhang,Liang Dong,Tiantian Zhou,Yi Wu,Ningzheng Dong,Qingyu Wu

Journal of cell science 130:2747-2756 PubMed28687622

2017

Polyphosphate as a donor of high-energy phosphate for the synthesis of ADP and ATP.

Applications

Unspecified application

Species

Unspecified reactive species

Werner E G Müller,Shunfeng Wang,Meik Neufurth,Maria Kokkinopoulou,Qingling Feng,Heinz C Schröder,Xiaohong Wang

PloS one 5:e13130 PubMed20976190

2010

Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.

Applications

ICC/IF

Species

Human

Tyler M Sharp,Susana Guix,Kazuhiko Katayama,Sue E Crawford,Mary K Estes

The journal of gene medicine 7:354-65 PubMed15619290

2004

Alteration in the IL-2 signal peptide affects secretion of proteins in vitro and in vivo.

Applications

ELISA

Species

Human

Lei Zhang,Qixin Leng,A James Mixson

International journal of cancer. Supplement = Journal international du cancer. Supplement 2:59-66 PubMed3162446

1988

Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.

Applications

Unspecified application

Species

Unspecified reactive species

H Durbin,E M Milligan,S J Mather,D F Tucker,R Raymond,W F Bodmer

International journal of cancer. Supplement = Journal international du cancer. Supplement 2:50-8 PubMed2450848

1988

Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.

Applications

Unspecified application

Species

Unspecified reactive species

H Durbin,D F Tucker,E M Milligan,L G Bobrow,P H Warne,Y L Pookim,W F Bodmer

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Placental alkaline phosphatase (PLAP) 抗体 [8B6]

Anti-Placental alkaline phosphatase (PLAP) antibody [8B6]

Western blot - Anti-Placental alkaline phosphatase (PLAP) antibody [8B6] (AB33)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise