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    products/primary-antibodies/pla2r-antibody-ab80054.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Lipases
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Anti-PLA2R 抗体 (ab80054)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (4)

Product price, shipping and contact information

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Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Rabbit polyclonal to PLA2R
  • Suitable for: IHC-Fr, WB
  • Reacts with: Human
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-PLA2R antibody
    PLA2R 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to PLA2R
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Fr, WBmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Rabbit, Cow
  • 免疫原

    Synthetic peptide derived from the C terminal domain of human PLA2R

  • 特記事項

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3173938-1, GR3173938-3, GR3173938-4, GR3173938-5, GR306201-4, GR306201-5, GR3173938-2, GR306201-3, GR306201-2, GR281777-4. Lot numbers other than GR3173938-1, GR3173938-3, GR3173938-4, GR3173938-5, GR306201-4, GR306201-5, GR3173938-2, GR306201-3, GR306201-2, GR281777-4 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
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    • Lipid metabolism
    • Metabolism
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    • Lipases
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    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab80054の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr (1)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
  • 組織特異性

    Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
  • 配列類似性

    Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • ドメイン

    C-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
    The endocytosis signal probably mediates endocytosis via clathrin-coated pits.
  • 翻訳後修飾

    The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q13018 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 317777 Cow
    • Entrez Gene: 22925 Human
    • Entrez Gene: 100009180 Rabbit
    • Omim: 604939 Human
    • SwissProt: P49259 Cow
    • SwissProt: Q13018 Human
    • SwissProt: P49260 Rabbit
    • Unigene: 410477 Human
    • 別名

      • 180 kDa secretory phospholipase A2 receptor antibody
      • C-type lectin domain family 13 member C antibody
      • CLEC13C antibody
      • M type receptor antibody
      • M-type receptor antibody
      • Phospholipase A2 receptor 1 antibody
      • PLA2 R antibody
      • PLA2-R antibody
      • PLA2G1R antibody
      • PLA2IR antibody
      • PLA2R antibody
      • PLA2R_HUMAN antibody
      • PLA2R1 antibody
      • Soluble PLA2-R antibody
      • Soluble PLA2R antibody
      • Soluble secretory phospholipase A2 receptor antibody
      see all

    プロトコール

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (4)

    ab80054 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80054 は 4 報の論文で使用されています。

    • Ramachandran R  et al. Membranous Nephropathy With Light Chain Restricted Deposits. Nephrology (Carlton) N/A:N/A (2018). PubMed: 29633425
    • Kumar V  et al. Primary membranous nephropathy in adolescence: A prospective study. Nephrology (Carlton) 22:678-683 (2017). PubMed: 27334445
    • Ramachandran R  et al. PLA2R antibodies, glomerular PLA2R deposits and variations in PLA2R1 and HLA-DQA1 genes in primary membranous nephropathy in South Asians. Nephrol Dial Transplant 31:1486-93 (2016). PubMed: 26673907
    • Kumar V  et al. Antibodies to m-type phospholipase A2 receptor in children with idiopathic membranous nephropathy. Nephrology (Carlton) 20:572-5 (2015). PubMed: 26194981

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-PLA2R antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Renal biopsy (fresh frozen))
    Permeabilization
    No
    Specification
    Renal biopsy (fresh frozen)
    Fixative
    MTM
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ashwani Kumar Verma

    Verified customer

    投稿 Aug 06 2015

    Question

    Do you have a recommendation for a control to test the PLA2R antibody?



    Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 19 2012

    Answer

    Thank you for contacting us. Kidney would be a good control for this product. Please let me know if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Dec 19 2012

    Question

    Product code: 80054

    Inquiry: Hi, I am looking for an anti-PLA2R antibody for mouse. I see you have one that you say is reactive to human, and is predicted to react with cow and rabbit. Do you know if it is also reactive with mouse PLA2R, or if you have products that do? Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 16 2012

    Answer

    Thank you for contacting us and your interest in our products.

    Unfortunately, we do not currently have any antibodies directed against PLA2R which have shown to detect the protein in mouse samples. I have checked the immunogen used to raise the two antibodies we have against this target (ab80054 and ab99466). Both unfortunately share very low sequence homology with the mouse protein, we would therefore not expect the antibodies to cross react.

    I am sorry that I could not be of more help in this instance. If you have any further questions, please do not hesitate to ask.

    Read More

    Abcam Scientific Support

    Answered on Aug 16 2012

    Question

    je vous contacte pour savoir si la concentration de l'anticorps anti-PLA2R (ab80054) que vous vendez est de 1mg/mL ?
    Nous vous avons acheté cet anticorps il y a une dizaine de jour et je ne trouve pas cette donnée dans la datasheet.
    Merci pour votre réponse,

    Read More

    Abcam community

    Verified customer

    Asked on May 16 2012

    Answer



    L'anticorps ab80054 est fourni sous forme non purifié (anti-sérum), sa concentration ne peut donc pas être déterminé. Pour information, la concentration d'anticorps dans unanti-sérum est estimée entre 1 et 10 mg/ml.

    Read More

    Abcam Scientific Support

    Answered on May 16 2012

    Question

    Dear Technical Team,



    Hope this e-mail finds you well!



    Kindly advise ifthe Anti-PLA2R antibody (ab80054) is suitable for serum samples as desired by the customer.



    Looking forward towards your valued response on it.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 09 2012

    Answer

    Thank you for your enquiry.

    With what specific application does your customer wish to use the antibody? And what species of serum are they working with?

    Thanks for the additional information.

    Read More

    Abcam Scientific Support

    Answered on Mar 09 2012

    Question

    I have a question about your pla2r antibodies.  The immunogen is stated to be in the c terminus for ab80054 but the immunogen is not specified.  Is it the same immunogen that is specified for your other PLA2r Ab (ab 99466)? Thank you,    

    Read More

    Abcam community

    Verified customer

    Asked on Jul 27 2011

    Answer

    Thank you for contacting us. I can confirm that the immunogen sequences for these two antibodies are different. The immunogen for ab99466 is from an internal domain (amino acid 946 - 957), whereas the immunogen for ab80054 is from a C terminal domain (exact sequence is however proprietary information). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jul 27 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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