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リコンビナントRabMAb

Anti-PKR (phospho T451) 抗体 [EPR2152Y] (ab81303)

Reviews (1)Q&A (2)References (27)
Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
  • Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
  • Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
  • Dot Blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
  • Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2152Y] to PKR (phospho T451)
  • Suitable for: Dot blot, WB
  • Reacts with: Human, Pig

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PKR (phospho T451) antibody [EPR2152Y]
    PKR 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2152Y] to PKR (phospho T451)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, WBmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human, Pig

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR2152Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab81303の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
WB (1)
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
特記事項
Dot blot
1/1000.
WB
Use at an assay dependent concentration. Detects a band of approximately 68 kDa (predicted molecular weight: 62 kDa).
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Double stranded RNA activated protein kinase; antibody
      • E2AK2_HUMAN antibody
      • eIF-2A protein kinase 2 antibody
      • EIF2AK1 antibody
      • EIF2AK2 antibody
      • Eukaryotic translation initiation factor 2 alpha kinase 2 antibody
      • Eukaryotic translation initiation factor 2-alpha kinase 2 antibody
      • HGNC:9437 antibody
      • Interferon induced double stranded RNA activated protein kinase antibody
      • Interferon inducible elF2 alpha kinase antibody
      • Interferon inducible RNA dependent protein kinase antibody
      • Interferon-induced, double-stranded RNA-activated protein kinase antibody
      • Interferon-inducible RNA-dependent protein kinase antibody
      • MGC126524 antibody
      • P1/eIF-2A protein kinase antibody
      • P1/eIF2A protein kinase antibody
      • p68 kinase antibody
      • PKR antibody
      • PPP1R83 antibody
      • PRKR antibody
      • Protein kinase interferon inducible double stranded RNA dependent antibody
      • Protein kinase RNA activated antibody
      • Protein kinase RNA-activated antibody
      • Protein phosphatase 1 regulatory subunit 83 antibody
      • Serine/threonine protein kinase TIK antibody
      • Tyrosine protein kinase EIF2AK2 antibody
      see all

    画像

    • Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma) treated with Interferon-a and Calyculin A whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 62 kDa



      Purified format.

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 0.048 µg/ml

      Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HeLa treated with 1000U/ml IFN a1 for 18 hours and then treated with 100nM Calyculin A for 15 minutes whole cell lysate
      Lane 3 : HeLa treated with 1000U/ml IFN a1 for 18 hours and then treated with 100nM Calyculin A for 15 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.01 µg/ml

      Predicted band size: 62 kDa



      Blocking/Diluting Buffer and concentration: 5% NFDM /TBST

    • Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      Western blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      All lanes : Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303) at 1/2000 dilution (undiluted)

      Lane 1 : HeLa cell lysate un-treated
      Lane 2 : HeLa cell lysate treated with IFN-alpha

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 62 kDa
      Observed band size: 68 kDa why is the actual band size different from the predicted?

    • Dot Blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      Dot Blot - Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

      Dot blot analysis of PKR (pT451) phospho peptide (Lane 1), PKR non-phospho peptide (Lane 2), labeling PKR (phospho T451) with ab81303 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes

    • Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)
      Anti-PKR (phospho T451) antibody [EPR2152Y] (ab81303)

    参考文献 (27)

    ab81303 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab81303 は 27 報の論文で使用されています。

    • Li H  et al. Regulation of PKR-dependent RNA translation inhibition by TRIM21 upon virus infection or other stress. PLoS Pathog 19:e1011443 (2023). PubMed: 37327222
    • Lin W  et al. Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment. J Immunother Cancer 11:N/A (2023). PubMed: 37935565
    • Price AM  et al. Adenovirus prevents dsRNA formation by promoting efficient splicing of viral RNA. Nucleic Acids Res 50:1201-1220 (2022). PubMed: 34671803
    • Lee S  et al. PKR-Mediated Phosphorylation of eIF2a and CHK1 Is Associated with Doxorubicin-Mediated Apoptosis in HCC1143 Triple-Negative Breast Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36555509
    • Zhu T  et al. GADD34-mediated dephosphorylation of eIF2α facilitates pseudorabies virus replication by maintaining de novo protein synthesis. Vet Res 52:148 (2021). PubMed: 34930429
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-PKR (phospho T451) antibody [EPR2152Y]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lung epithelial)
    Loading amount
    10 µg
    Specification
    Lung epithelial
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 18 2011

    Question

    What is the specificity of this antibody towards T451 or T456?

    Read More
    Answer

    Thank you for contacting us.

    The antibody is specific to phosphorylation site of T451, the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    wir haben bei Ihnen einen Antikörper bestellt (über unsere Krankenhausapotheke).





    PKR ab81303 Rabbit monoclonal (phospho T451).

    Dieser kam gestern Nachmittag warm an. Der Kühl- Gefrierakku war ueber 20C Da dieser AK nicht ganz unkompliziert in der Anwendung ist,

    möchten wir gerne wissen, ob er noch in Ordnung ist.

    Read More
    Answer

    Der Antikörper sollte dies eigentlich unbeschadet überstanden haben. Wir versenden unsere Antikörper so z.B. auch nach Australien oder Thailand, und da kann es schon einmal zu Transitzeiten von einer Woche kommen, und bisher haben wir noch keine erhöhten Beschwerderaten für die so versandten Produkte.


    Die meisten Antikörper sind für längere Zeit bei Raumtemperatur stabil. Versuche bei Abcam mit Antikörpern, die über einen längeren Zeitraum bei Raumtemperatur gelagert wurden, haben gezeigt, dass die Aktivität erst nach zwei Monaten nachlässt. Dennoch verschicken wir Antikörper als Vorsichtsmaßnahme mit Kühlpaketen, die die Umgebungstemperatur für 48 Stunden bei circa 4°C halten. Dieses verbessert auch die Haltbarkeit der Antikörper und verringert das rapide Auf- und Abkühlen der Antikörper.

    Falls es widererwartend doch zu Problemem kommt, melden sie sich bitte bei uns.

    Read More

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