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リコンビナントRabMAb

Anti-PKD2 (phospho S876) 抗体 [EP1496Y] (ab51251)

Reviews (4) Submit a question References (3)
Immunoprecipitation - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
  • Western blot - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
  • Western blot - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
  • Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1496Y] to PKD2 (phospho S876)
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PKD2 (phospho S876) antibody [EP1496Y]
    PKD2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1496Y] to PKD2 (phospho S876)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa treated with 100nM calyculin A, HEK-293 treated with 100nM calyculin A IP: ab51251 + HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate.

  • 特記事項

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP1496Y

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab51251の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
IP
1/20.
特記事項
WB
1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
IP
1/20.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.

  • 細胞内局在

    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

画像

  • Immunoprecipitation - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
    Immunoprecipitation - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)

    Purified ab51251 at 1:20 dilution (0.6µg) immunoprecipitating PKD2 in HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate.
    Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 10µg.
    Lane 2 (+): ab51251 + HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51251 in HEK-293 treated with 50nM Calyculin A for 3 hours whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP)™(ab131366) (1:1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 105 kDa

  • Western blot - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
    Western blot - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
    All lanes : Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution (Purified)

    Lane 1 : untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight and treated with 100nM calyculin A for 60 minutes whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight and treated with 100nM calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
    Western blot - Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
    All lanes : Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution (Purified)

    Lane 1 : untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight and treated with 100nM calyculin A for 60 minutes, whole cell lysate
    Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight and treated with 100nM calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 97 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?

  • Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)
    Anti-PKD2 (phospho S876) antibody [EP1496Y] (ab51251)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (3)

ab51251 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab51251 は 3 報の論文で使用されています。

  • Liu Q  et al. PRKD2 Promotes Progression and Chemoresistance of AML via Regulating Notch1 Pathway. Onco Targets Ther 12:10931-10941 (2019). PubMed: 31849496
  • Matsumura K  et al. Autism-associated protein kinase D2 regulates embryonic cortical neuron development. Biochem Biophys Res Commun 519:626-632 (2019). PubMed: 31540692
  • Bernhart E  et al. Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro. Exp Cell Res 319:2037-48 (2013). PubMed: 23562655

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-PKD2 (phospho S876) antibody [EP1496Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (BV2 microglia)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Treatment
1µM LPA for 40min in the absence or presence of a PKD family inhibitor (CRT1µM)
Specification
BV2 microglia
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Lisha Joshi

Verified customer

投稿 Jun 12 2019

Western blot abreview for Anti-PKD2 (phospho S876) antibody [EP1496Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary murine microglia)
Gel Running Conditions
Reduced Non-Denaturing (Native) (7%)
Loading amount
100 µg
Treatment
Lysophosphatidic acid (LPA) 1µM
Specification
Primary murine microglia
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Joanna Plastira

Verified customer

投稿 Oct 12 2018

Western blot abreview for Anti-PKD2 (phospho S876) antibody [EP1496Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (Left ventricle)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Left ventricle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Christopher Trummer

Verified customer

投稿 Oct 04 2018

Western blot abreview for Anti-PKD2 (phospho S876) antibody [EP1496Y]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Left Ventricle)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Left Ventricle
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Christopher Trummer

Verified customer

投稿 Oct 04 2018

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