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リコンビナントRabMAb

Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) 抗体 [EP1491Y] (ab76129)

Submit a review Submit a question References (6)
Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
  • Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC iota (phospho T412)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y]

  • 製品の詳細

    Rabbit monoclonal [EP1491Y] to PKC zeta (phospho T410) + PKC iota (phospho T412)

  • 由来種

    Rabbit

  • 特異性

    This antibody detects PKC zeta and PKC lamda phosphorylated at T410/412 respectively.

    This antibody also detects various PKC phosphorylated isoforms.

  • アプリケーション

    適用あり: IHC-P, WBmore details
    適用なし: IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293 cell lysate, human brain tissue; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76129の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
1/20000. Predicted molecular weight: 68 kDa.

For unpurified, use 1/1000 - 1/2000.
特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
1/20000. Predicted molecular weight: 68 kDa.

For unpurified, use 1/1000 - 1/2000.
追加情報
Is unsuitable for IP.

ターゲット情報

画像

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    All lanes : Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/1000 dilution

    Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
    Lane 2 : Recombinant human PKC beta 1 protein (ab60840)
    Lane 3 : Recombinant human PKC beta 2 protein (ab60841)
    Lane 4 : Recombinant human PKC gamma protein (ab60842)
    Lane 5 : Recombinant human PKC delta protein (ab60844)
    Lane 6 : Recombinant human PKC epsilon protein (ab60847)
    Lane 7 : Recombinant human PKC zeta protein (ab60848)
    Lane 8 : Recombinant human PKC eta protein (ab60849)
    Lane 9 : Recombinant human PKC theta/PRKCQ protein (ab56641)
    Lane 10 : Recombinant human PKC iota protein (ab60850)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 68 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 180 seconds; Lane 5-6: 60 seconds; Lane 7-10: 5 seconds.

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified) + Mouse lung tissue lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    All lanes : Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution (unpurified)

    Lane 1 : 293 cell lysates, untreated
    Lane 2 : 293 cell lysates, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit-HRP at 1/1000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa
    Additional bands at: 165 kDa, 170 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Western blot - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    All lanes : Anti-PKC zeta (phospho T410) + PKC iota (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified)

    Lane 1 : Untreated HeLa cell lysate
    Lane 2 : HeLa cell lysate treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 68 kDa
    Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

    Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)
    Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129)

参考文献 (6)

ab76129 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76129 は 6 報の論文で使用されています。

  • Zhang F  et al. PKC-ζ mediated reduction of the extracellular vesicles-associated TGF-β1 overcomes radiotherapy resistance in breast cancer. Breast Cancer Res 25:38 (2023). PubMed: 37029374
  • Valdivia A  et al. Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization. Front Cell Dev Biol 11:1231489 (2023). PubMed: 37635877
  • Liu S  et al. Lonicera caerulea Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation. Front Nutr 9:853225 (2022). PubMed: 35356725
  • Chau DD  et al. Attenuation of amyloid-β generation by atypical protein kinase C-mediated phosphorylation of engulfment adaptor PTB domain containing 1 threonine 35. FASEB J 33:12019-12035 (2019). PubMed: 31373844
  • Yeo MK  et al. Phosphorylated Protein Kinase C (Zeta/Lambda) Expression in Colorectal Adenocarcinoma and Its Correlation with Clinicopathologic Characteristics and Prognosis. J Cancer 8:3371-3377 (2017). PubMed: 29158810
  • Raychaudhuri S MicroRNAs Overexpressed in Growth-Restricted Rat Skeletal Muscles Regulate the Glucose Transport in Cell Culture Targeting Central TGF-ß Factor SMAD4. PLoS One 7:e34596 (2012). WB ; Rat . PubMed: 22506032

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