Anti-PKC theta/PRKCQ (phospho S676) 抗体 (ab47774)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ (phospho S676)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC theta/PRKCQ (phospho S676) antibody
PKC theta/PRKCQ 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC theta/PRKCQ (phospho S676) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PKC theta/PRKCQ aa 600-700 (C terminal) (phospho S676).
Database link: Q04759 -
ポジティブ･コントロール
- IHC-P: Human breast carcinoma. WB: JK cell extract treated with 200nM PMA for 30mins.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47774の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
組織特異性
Skeletal muscle, megakaryoblastic cells and platelets. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
ドメイン
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻訳後修飾
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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参照データベース
- Entrez Gene: 5588 Human
- Omim: 600448 Human
- SwissProt: Q04759 Human
- Unigene: 498570 Human
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別名
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)This image shows human breast carcinoma stained with ab47774 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phosphopeptide, whereas the left hand image is untreated.
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Western blot - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)All lanes : Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774) at 1/500 dilution
Lane 1 : JK cell extract treated with 200nM PMA for 30mins. No peptide.
Lane 2 : JK cell extract, untreated. No peptide.
Predicted band size: 82 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab47774 は 2 報の論文で使用されています。
- Li J et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci 129:2448-61 (2016). WB ; Human . PubMed: 27149922
- Aguiló JI et al. Protein kinase C-theta is required for NK cell activation and in vivo control of tumor progression. J Immunol 182:1972-81 (2009). WB ; Mouse . PubMed: 19201850