Anti-PKC theta/PRKCQ 抗体 (ab185974)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC theta/PRKCQ antibody
PKC theta/PRKCQ 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC theta/PRKCQ -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human PKC theta/PRKCQ aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_006248.1 -
ポジティブ･コントロール
- Jurkat whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185974の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
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| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 82 kDa.
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| 特記事項 |
|---|
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IP
Use at 2-10 µg/mg of lysate. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 82 kDa. |
ターゲット情報
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機能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
組織特異性
Skeletal muscle, megakaryoblastic cells and platelets. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
ドメイン
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻訳後修飾
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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参照データベース
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
see all -
別名
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
画像
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Immunoprecipitation - Anti-PKC theta/PRKCQ antibody (ab185974)
Detection of PKC theta/PRKCQ by Western Blot of Immunoprecipitated jurkat whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185974, for IP at 6 µg/mg lysate and for blotting at 1 µg/ml.
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Western blot - Anti-PKC theta/PRKCQ antibody (ab185974)Anti-PKC theta/PRKCQ antibody (ab185974) at 0.1 µg/ml + Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 82 kDa
Exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185974 は 1 報の論文で使用されています。
- Dybas JM et al. Integrative proteomics reveals an increase in non-degradative ubiquitylation in activated CD4+ T cells. Nat Immunol 20:747-755 (2019). PubMed: 31061531