AB109539

Anti-PKC (phospho T514) 抗体 [EP2730Y]

Anti-PKC (phospho T514) antibody [EP2730Y]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(24 Publications)

Rabbit Recombinant Monoclonal KPCG phospho T514 antibody. Suitable for IHC-P, IP, WB, I-ELISA, Dot and reacts with Mouse, Rat, Human, Synthetic peptide - Human, Synthetic peptide samples. Cited in 24 publications.

別名を表示する

PKCG, PRKCG, Protein kinase C gamma type, PKC-gamma

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebral cortex tissue sections labeling PKC with Purified ab109539 at 1 : 300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Unpurified ab109539 at 1/100 dilution staining PKC (phospho T514) in paraffin-embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

I-ELISA

Lab

Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Various phosphorylated PKC peptides at 1000 ng/ml stained for PKC (phospho T514) using ab109539 at 0-1000 ng/ml in ELISA. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as a secondary antibody at a concentration of 1/2500.

Antigen :
Human PKC gamma (pT514)
Human PKC alpha (pT497)
Human PKC beta (pT500)
Human PKC delta (pT507)
Human PKC eta (pT513)
Human PKC epsilon (pT566)
Human PKC theta (pT538)
Human PKC gamma non-phospho

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebellum tissue sections labeling PKC with Purified ab109539 at 1 : 300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebellum tissue sections labeling PKC with Purified ab109539 at 1 : 300 dilution (1.14 μg/ml). Heat mediated antigen retrieval was performed using citrate buffer pH 6.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Unknown

Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

ab109539 (purified) at 1 : 20 dilution (2μg) immunoprecipitating PKC in Rat brain lysate.
Lane 1 (input) : Rat brain lysate 10μg
Lane 2 (+) : ab109539 & Rat brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab109539 in Rat brain lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

Predicted band size: 77 kDa

false

Lab

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 0.02 µg/mL

Lane 1:

HeLa cell lysate with PKC gamma (pT514) peptide

Lane 2:

HeLa cell lysate with PKC gamma non-phospho peptide

Lane 3:

HeLa cell lysate

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Blocking and dilution buffer : 5% NFDM/TBST.

ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).

Active human PKC alpha full length protein (Catalog#ab55672) contains aa1-672 with GST-tag;
Active human PKC beta 1 full length protein (Catalog#ab60840) contains aa1-671 with GST-tag;
Active human PKC beta 2 full length protein (Catalog#ab60841) contains aa1-673 with GST-tag;
Active human PKC delta full length protein (Catalog#ab60844) contains aa1-676 with GST-tag;
Active human PKC eta full length protein (Catalog#ab60849) contains aa1-683 with GST-tag;
Active human PKC epsilon full length protein (Catalog#ab60847) contains aa1-737 with GST-tag;
Active human PKC theta full length protein (Catalog#ab56641) contains aa1-706 with GST-tag;
Active human PKC mu full length protein (Catalog#ab60873) contains aa1-912 with GST-tag;
Active human PKC gamma full length protein (Catalog#ab60842) contains aa1-697 with GST-tag.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/1000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>) at 0.5 µg

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>) at 0.2 µg

Lane 3:

Active human PKC beta 2 full length protein at 0.2 µg

Lane 4:

Western blot - Recombinant human PKC delta protein (<a href='/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>) at 0.05 µg

Lane 5:

Western blot - Recombinant human PKC eta protein (<a href='/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>) at 0.5 µg

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>) at 1 µg

Lane 7:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>) at 0.2 µg

Lane 8:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>) at 1 µg

Lane 9:

Western blot - Recombinant human PKC gamma protein (<a href='/products/proteins-peptides/recombinant-human-pkc-gamma-protein-ab60842'>ab60842</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate whole cell lysates, the membrane was then incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

Lab

Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Various phosphorylated PKC peptides stained for PKC (phospho T514) using ab109539 at 0.543 μg/ml in Dot blot. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at a dilution of 1/100,000. Exposure time : 180 seconds.

Blocking and dilution buffer : 5% NFDM/TBST.

Lane 1 : Human PKC gamma (pT514) peptide
Lane 2 : Human PKC alpha (pT497) peptide
Lane 3 : Human PKC gamma non-phospho peptide
Lane 4 : Human PKC beta (pT500) peptide
Lane 5 : Human PKC delta (pT507) peptide
Lane 6 : Human PKC eta (pT513) peptide
Lane 7 : Human PKC epsilon (pT566) peptide
Lane 8 : Human PKC theta (pT538) peptide

ab109539 could detect PKC gamma (pT514), PKC alpha (pT497), PKC beta (pT500), PKC delta (pT507), PKC eta (pT513), PKC epsilon (pT566) and PKC theta (pT538).

All lanes:

Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539)

Lane 1:

Human PKC gamma (pT514) peptide

Lane 2:

Human PKC alpha (pT497) peptide

Lane 3:

Human PKC gamma non-phospho peptide

Lane 4:

Human PKC beta (pT500) peptide

Lane 5:

Human PKC delta (pT507) peptide

Lane 6:

Human PKC eta (pT513) peptide

Lane 7:

Human PKC epsilon (pT566) peptide

Lane 8:

Human PKC theta (pT538) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Unknown

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/2000 dilution

Lane 1:

Rat brain lysates at 15 µg

Lane 2:

Rat brain lysates and then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

false

Unknown

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (ab109539) at 1/2000 dilution

Lane 1:

Mouse cerebellum lysates at 15 µg

Lane 2:

Mouse cerebellum lysates and then the membrane was incubated with phosphatase. at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2730Y

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, I-ELISA, IHC-P, Dot, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "For unpurified use at 1/100 - 1/250. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1/50.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress (By similarity). Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock (By similarity). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).

See full target information PRKCG phospho T514

style

left-column

文献 (24)

Recent publications for all applications. Explore the full list and refine your search

BMJ open diabetes research & care 13: PubMed40716951

2025

Glucose absorption in the duodenum is modulated by an estrogen receptor α-dependent regulation of glucose transporter functional expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Ding,Xiaoxu Yang,Weixi Shan,Jingyu Xu,Qian Du,Changmei Chen,Qiushi Liao,Jun Lou,Zhe Jin,Mingkai Chen,Rui Xie

Journal of pharmaceutical analysis 14:101149 PubMed39720622

2024

Depression of Ca1.2 activation and expression in mast cells ameliorates allergic inflammation diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjing Zhang,Yingnan Zeng,Haoyun Bai,Wen Zhang,Zhuoyin Xue,Shiling Hu,Shemin Lu,Nan Wang

International journal of biological sciences 20:3956-3971 PubMed39113702

2024

PKCα Induced the Generation of Extracellular Vesicles in Activated Platelets to Promote Breast Cancer Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghua Zhao,Huan Tian,Xiaona Zhao,Lan Lan,Huanhuan Liu,Yi Sun,Fengyan Yu

CNS neuroscience & therapeutics 29:3644-3656 PubMed37302990

2023

Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Ma,Qingyuan Wu,Jinxian Yuan,You Wang,Peng Zhang,Qiankun Liu,Dandan Tan,Minxue Liang,Yangmei Chen

Cellular & molecular biology letters 28:16 PubMed36849880

2023

The RXFP2-PLC/PKC signaling pathway mediates INSL3-induced regulation of the proliferation, migration and apoptosis of mouse gubernacular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shouxing Duan,Xuewu Jiang,Jianhong Li,Maxian Fu,Zhuo Li,Yiyi Cheng,Yangmu Zhuang,Ming Yang,Wenfeng Xiao,Hongyan Ping,Yao Xie,Xiaojun Xie,Xuan Zhang

Journal of neuroscience research 101:1170-1187 PubMed36807930

2023

Metabotropic glutamate receptor 5-mediated inhibition of inward-rectifying K channel 4.1 contributes to orofacial ectopic mechanical allodynia following inferior alveolar nerve transection in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ke Li,Yan-Yan Zhang,Jiu Lin,Ya-Jing Liu,Yue-Ling Li,Yu-Heng Feng,Jia-Shuo Zhao,Cheng Zhou,Fei Liu,Jie-Fei Shen

Translational cancer research 11:2050-2060 PubMed36249885

2022

Effect of IL-8 on hepatocellular carcinoma-associated metastasis by targeting MMP9 in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Chao Xue,Han-Yu Zhou,Liu-Shen-Yan Yu,Yan-Yan Wu

Molecular neurobiology 59:5179-5192 PubMed35678978

2022

Celsr3 Inactivation in the Brainstem Impairs Rubrospinal Tract Development and Mouse Behaviors in Motor Coordination and Mechanic-Induced Response.

Applications

Unspecified application

Species

Unspecified reactive species

Boli Chen,Fuxiang Li,Bin Jia,Kwok-Fai So,Ji-An Wei,Yuchu Liu,Yibo Qu,Libing Zhou

Environmental toxicology 37:910-924 PubMed34995009

2022

Inhibition of ALKBH5-mediated m A modification of PPARG mRNA alleviates H/R-induced oxidative stress and apoptosis in placenta trophoblast.

Applications

Unspecified application

Species

Unspecified reactive species

Yongping Guo,Wenxia Song,Yali Yang

Annals of translational medicine 9:1470 PubMed34734022

2021

Cold-inducible RNA-binding protein migrates from the nucleus to the cytoplasm under cold stress in normal human bronchial epithelial cells via TRPM8-mediated mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Ping Mao,Yan Jiao,Jian-Hua Xiang,Xin-Wei Luo,Qian He,Dan-Hua Ran,Qing Xu,Chun-Hui Lang,Ling-Xiu Chen

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKC (phospho T514) 抗体 [EP2730Y]

Anti-PKC (phospho T514) antibody [EP2730Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Indirect ELISA - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Immunoprecipitation - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Dot Blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

Western blot - Anti-PKC (phospho T514) antibody [EP2730Y] (AB109539)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (24)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise