AB75837

Anti-PKC (phospho S660) 抗体 [EP1902Y]

Name: Anti-PKC (phospho S660) 抗体 [EP1902Y] (ab75837) | Abcam
Brand: Abcam Limited
SKU: AB75837
Price: 42000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-PKC (phospho S660) antibody [EP1902Y]

RabMAb
詳細を見る

(1 Review)

(17 Publications)

Rabbit Monoclonal KPCA phospho S660 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 17 publications.

別名を表示する

PKCA, PRKACA, PRKCA, Protein kinase C alpha type, PKC-A, PKC-alpha

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

ab75837, at a 1/100 dilution, staining PKC in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Lab

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/5000 dilution

Lane 1:

HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysates at 15 µg

Lane 2:

HEK293 (Human epithelial cell line from embryonic kidney) treated with Phorbol-12-myristate-13-acetate at 100nM for 1 hour whole cell lysates at 15 µg

Lane 3:

HEK293 (Human epithelial cell line from embryonic kidney) treated with Phorbol-12-myristate-13-acetate at 100nM for 1 hour whole cell lysates. Then the membrane was incubated with phosphatas at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Blocking and dilution buffer : 5% NFDM/TBST.

Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag.
Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag.
Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag.
Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag.
Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag.
Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag.
Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag.
Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

All lanes:

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/20000 dilution

Lane 1:

Western blot - Recombinant human PKC alpha protein (Active) (<a href='/products/proteins-peptides/recombinant-human-pkc-alpha-protein-active-ab55672'>ab55672</a>)

Lane 2:

Western blot - Recombinant human PKC beta 1 protein (<a href='/products/proteins-peptides/recombinant-human-pkc-beta-1-protein-ab60840'>ab60840</a>)

Lane 3:

Western blot - Recombinant human PKC beta 2 protein (<a href='/products/proteins-peptides/recombinant-human-pkc-beta-2-protein-ab60841'>ab60841</a>)

Lane 4:

Western blot - Recombinant human PKC delta protein (<a href='/products/proteins-peptides/recombinant-human-pkc-delta-protein-ab60844'>ab60844</a>)

Lane 5:

Western blot - Recombinant human PKC eta protein (<a href='/products/proteins-peptides/recombinant-human-pkc-eta-protein-ab60849'>ab60849</a>)

Lane 6:

Western blot - Recombinant human PKC epsilon protein (<a href='/products/proteins-peptides/recombinant-human-pkc-epsilon-protein-ab60847'>ab60847</a>)

Lane 7:

Western blot - Recombinant human PKC theta/PRKCQ protein (<a href='/products/proteins-peptides/recombinant-human-pkc-theta-prkcq-protein-ab56641'>ab56641</a>)

Lane 8:

Western blot - Recombinant human PKC mu/PKD protein (<a href='/products/proteins-peptides/recombinant-human-pkc-mu-pkd-protein-ab60873'>ab60873</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 100-150 kDa

false

Exposure time: 1s

Unknown

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

All lanes:

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/100000 dilution

Lane 1:

Jurkat cell lysates, untreated at 10 µg

Lane 2:

Jurkat cell lysates, treated with AP at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

Lab

Dot Blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Dot blot analysis of PKC beta 2 (phospho S660) phospho peptide (Lane 1), PKC beta 2 Non-phospho peptide (Lane 2), labelling PKC (phospho S660) with ab75837 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP1902Y

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

Dot, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

ab75837 detects various isoforms of PKC phosphorylated.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Protein kinase C (PKC) comprises a family of serine/threonine kinases with several isoforms such as PKC alpha beta and gamma among others. PKC enzymes play key roles in intracellular signaling particularly in mediating responses to growth factors hormones and other signals. PKC exhibits a molecular weight generally ranging around 77 to 97 kDa depending on the isoform. These enzymes show expression in many tissues but have high concentrations in the brain heart and lung. PKC activation often involves translocation to cellular membranes which is important for their signaling roles.

Biological function summary

PKC influences various cellular processes such as cell proliferation differentiation apoptosis and immune responses. It acts within larger protein complexes serving as a modulator of cellular functions. The activation of PKC leads to its association with different scaffolding proteins impacting a wide array of cell activities. PKC notably affects the function of other kinases and transcription factors demonstrating its integral role in managing cellular behavior and homeostasis.

Pathways

PKC participates in the phosphoinositide signaling pathway and the mitogen-activated protein kinase (MAPK) pathway. In these contexts PKC activation impacts proteins like Ras and Raf kinases which play roles in cellular growth and differentiation. By modulating these pathways PKC becomes an important component in signal transduction cascades influencing cell fate decisions. Understanding the interaction between PKC and these pathways helps in elucidating its role in maintaining normal cellular activities and responses to external stimuli.

PKC is linked to cancer and cardiovascular diseases. In cancer dysregulation of PKC activity often contributes to tumor growth and progression by altering pathways that control cell division and survival. PKC abnormally interacts with proteins like Bcl-2 promoting cell survival. In cardiovascular diseases changes in PKC signaling can impact heart function contributing to conditions like heart failure through interactions with proteins such as troponin. Research on PKC and these diseases can provide insights into developing targeted therapies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. During chemokine-induced CD4(+) T cell migration, phosphorylates CDC42-guanine exchange factor DOCK8 resulting in its dissociation from LRCH1 and the activation of GTPase CDC42 (PubMed : 28028151). Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates SOCS2 at 'Ser-52' facilitating its ubiquitination and proteasomal degradation (By similarity). Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (PubMed : 25313067). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (PubMed : 36040231).

See full target information PRKCA phospho S660

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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed39836470

2025

Map2k6 is a potent genetic modifier of arterial rupture in vascular Ehlers-Danlos syndrome mice.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin J Bowen,Rebecca Sorber,Juan Francisco Calderón Giadrosic,Jefferson J Doyle,Graham Rykiel,Zachary Burger,Xiaoyan Zhang,Wendy A Espinoza Camejo,Nicole Anderson,Simone Sabnis,Chiara Bellini,Elena Gallo MacFarlane,Harry C Dietz

Molecular cell 84:4774-4789.e9 PubMed39591967

2024

The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Constance Vind,Zhenzhen Wu,Muhammad Jasrie Firdaus,Goda Snieckute,Gee Ann Toh,Malin Jessen,José Francisco Martínez,Peter Haahr,Thomas Levin Andersen,Melanie Blasius,Li Fang Koh,Nina Loeth Maartensson,John E A Common,Mads Gyrd-Hansen,Franklin L Zhong,Simon Bekker-Jensen

Cell death & disease 14:467 PubMed37495584

2023

Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Ryder,Frederic Schrøder Arendrup,José Francisco Martínez,Goda Snieckute,Chiara Pecorari,Riyaz Ahmad Shah,Anders H Lund,Melanie Blasius,Simon Bekker-Jensen

Cell metabolism 34:2036-2046.e8 PubMed36384144

2022

Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Goda Snieckute,Aitana Victoria Genzor,Anna Constance Vind,Laura Ryder,Mark Stoneley,Sébastien Chamois,René Dreos,Cathrine Nordgaard,Frederike Sass,Melanie Blasius,Aida Rodríguez López,Sólveig Hlín Brynjólfsdóttir,Kasper Langebjerg Andersen,Anne E Willis,Lisa B Frankel,Steen Seier Poulsen,David Gatfield,Zachary Gerhart-Hines,Christoffer Clemmensen,Simon Bekker-Jensen

Folia histochemica et cytobiologica 60:271-279 PubMed36177743

2022

Wnt5a/Ror2 promotes vascular smooth muscle cells proliferation via activating PKC.

Applications

Unspecified application

Species

Unspecified reactive species

Yaning Shi,Hongfang Li,Jia Gu,Yongzhen Gong,Xuejiao Xie,Duanfang Liao,Li Qin

PLoS genetics 18:e1010059 PubMed35245290

2022

Comparative therapeutic strategies for preventing aortic rupture in a mouse model of vascular Ehlers-Danlos syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Legrand,Charline Guery,Julie Faugeroux,Erika Fontaine,Carole Beugnon,Amélie Gianfermi,Irmine Loisel-Ferreira,Marie-Christine Verpont,Salma Adham,Tristan Mirault,Juliette Hadchouel,Xavier Jeunemaitre

International journal of biological sciences 17:2561-2575 PubMed34326694

2021

Celastrol ameliorates vascular neointimal hyperplasia through Wnt5a-involved autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ning Shi,Le-Ping Liu,Chang-Feng Deng,Tan-Jun Zhao,Zhe Shi,Jian-Ye Yan,Yong-Zhen Gong,Duan-Fang Liao,Li Qin

The Journal of clinical investigation 130:686-698 PubMed31639107

2019

Targetable cellular signaling events mediate vascular pathology in vascular Ehlers-Danlos syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin J Bowen,Juan Francisco Calderón Giadrosic,Zachary Burger,Graham Rykiel,Elaine C Davis,Mark R Helmers,Kelly Benke,Elena Gallo MacFarlane,Harry C Dietz

Biomedicine & pharmacotherapy = Biomedecine & phar 106:805-812 PubMed29990874

2018

Therapeutic effects of JLX001 on cerebral ischemia through inhibiting platelet activation and thrombus formation in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Yi Yan,Lu-Yao Ao,Lin Zhou,Cheng-Yuan Li,Wei-Rong Fang,Wei-Yang Shen,Bing-Wen Liang,Xiong Zhu,Yun-Man Li

Molecular neurobiology 56:1870-1882 PubMed29967987

2018

Wnt-7a Stimulates Dendritic Spine Morphogenesis and PSD-95 Expression Through Canonical Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Ramos-Fernández,Cheril Tapia-Rojas,Valerie T Ramírez,Nibaldo C Inestrosa

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-PKC (phospho S660) 抗体 [EP1902Y]

Anti-PKC (phospho S660) antibody [EP1902Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Dot Blot - Anti-PKC (phospho S660) antibody [EP1902Y] (AB75837)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (17)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise