Anti-PKC mu/PKD 抗体 [EP1493Y] (ab51246)
![Western blot - Anti-PKC mu/PKD antibody [EP1493Y] (ab51246)](https://www.abcam.co.jp/ps/products/51/ab51246/Images/ab51246-63194-anti-pkc-mu-antibody-ep1493y-western-blot.jpg "Western blot - Anti-PKC mu/PKD antibody [EP1493Y] (ab51246)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1493Y] to PKC mu/PKD
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-PKC mu/PKD antibody [EP1493Y]
PKC mu/PKD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1493Y] to PKC mu/PKD -
由来種
Rabbit -
特異性
The immunogen used for this product shares 100% homology with PKD2 and PKD3. Cross-reactivity with these proteins have not been confirmed experimentally.
-
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
-
免疫原
Synthetic peptide corresponding to Human PKC mu/PKD aa 700-800.
-
ポジティブ･コントロール
- HeLa cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1493Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51246の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 102 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 102 kDa). |
追加情報
Is unsuitable for Flow Cyt or IP.
ターゲット情報
-
機能
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
-
参照データベース
- Entrez Gene: 5587 Human
- Entrez Gene: 18760 Mouse
- Entrez Gene: 85421 Rat
- Omim: 605435 Human
- SwissProt: Q15139 Human
- SwissProt: Q62101 Mouse
- SwissProt: Q9WTQ1 Rat
- Unigene: 508999 Human
see all -
別名
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
画像
-
Western blot - Anti-PKC mu/PKD antibody [EP1493Y] (ab51246)All lanes : Anti-PKC mu/PKD antibody [EP1493Y] (ab51246) at 1/1000 dilution
Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with PMA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted? -
![Anti-PKC mu/PKD antibody [EP1493Y] (ab51246)](https://www.abcam.co.jp/ps/products/51/ab51246/Images/ab51246-2-benefits-of-recombinant-antibodies.png)
Anti-PKC mu/PKD antibody [EP1493Y] (ab51246)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab51246 は 4 報の論文で使用されています。
- Perveen K et al. Characterization of the Transient Deficiency of PKC Isozyme Levels in Immature Cord Blood T Cells and Its Connection to Anti-Allergic Cytokine Profiles of the Matured Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34884454
- Zhu Z et al. Inhibition of protein kinase D by CID755673 promotes maintenance of the pluripotency of embryonic stem cells. Development 147:N/A (2020). PubMed: 32747433
- Bonfim-Melo A et al. Trypanosoma cruzi extracellular amastigotes trigger the protein kinase D1-cortactin-actin pathway during cell invasion. Cell Microbiol 17:1797-810 (2015). PubMed: 26096820
- Sinnett-Smith J et al. Protein kinase D1 mediates stimulation of DNA synthesis and proliferation in intestinal epithelial IEC-18 cells and in mouse intestinal crypts. J Biol Chem 286:511-20 (2011). PubMed: 21051537