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リコンビナントRabMAb

Anti-PKC eta 抗体 [EPR18513] (ab179524)

Reviews (1) Submit a question References (5)
Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
  • Anti-PKC eta antibody [EPR18513] (ab179524)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18513] to PKC eta
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PKC eta antibody [EPR18513]
    PKC eta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18513] to PKC eta

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Active human PKC eta full length protein; Human fetal brain, fetal heart, fetal skin and fetal kidney lysates; MCF7 and PC-3 whole cell lysates; Mouse skin, lung and brain lysates; Rat skin and brain lysates. IP: Mouse lung whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR18513

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab179524の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Detects a band of approximately 78, 40 kDa (predicted molecular weight: 78 kDa).
IP
1/50.
特記事項
WB
1/2000. Detects a band of approximately 78, 40 kDa (predicted molecular weight: 78 kDa).
IP
1/50.

ターゲット情報

  • 機能

    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • 組織特異性

    Most abundant in lung, less in heart and skin.

  • 関連疾患

    Defects in PRKCH may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • ドメイン

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • KPCL_HUMAN antibody
    • MGC 5363 antibody
    • MGC26269 antibody
    • MGC5363 antibody
    • nPKC eta antibody
    • nPKC-eta antibody
    • PKC h antibody
    • PKC L antibody
    • PKC-L antibody
    • PKCh antibody
    • PKCL antibody
    • Prkch antibody
    • PRKCL antibody
    • Protein kinase C eta antibody
    • Protein kinase C eta type antibody
    see all

画像

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1 : Active human PKC alpha full length protein
    Lane 2 : Active human PKC beta 1 full length protein
    Lane 3 : Active human PKC beta 2 full length protein
    Lane 4 : Active human PKC gamma full length protein
    Lane 5 : Active human PKC delta full length protein
    Lane 6 : Active human PKC eta full length protein
    Lane 7 : Active human PKC epsilon full length protein
    Lane 8 : Active human PKC theta full length protein
    Lane 9 : Active human PKC mu full length protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein(ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.

  • Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (ab179524)
    Immunoprecipitation - Anti-PKC eta antibody [EPR18513] (ab179524)

    PKC eta was immunoprecipitated from 1mg of Mouse lung whole cell lysate with ab179524 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179524 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse lung whole cell lysate 10ug (Input).

    Lane 2: ab179524 IP in Mouse lung whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179524 in Mouse lung whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 45,78 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 45,78 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC. 1995. J. Biol. Chem. 270, 28495-28498)

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1 : Human fetal skin lysate
    Lane 2 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 45,78 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

     

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/2000 dilution

    Lane 1 : Rat skin lysate
    Lane 2 : Mouse skin lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 45,78 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

     

  • Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    Western blot - Anti-PKC eta antibody [EPR18513] (ab179524)
    All lanes : Anti-PKC eta antibody [EPR18513] (ab179524) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 78 kDa
    Observed band size: 45,78 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The antibody recognizes the full length (~78KD) and the catalytic domain (~40KD) of PKC eta.
    (Geraldes P and King GL, 2010. Circ Res. 106, 1319-1331; Newton AC, 1995. J. Biol. Chem. 270, 28495-28498)

     

  • Anti-PKC eta antibody [EPR18513] (ab179524)
    Anti-PKC eta antibody [EPR18513] (ab179524)

参考文献 (5)

ab179524 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab179524 は 5 報の論文で使用されています。

  • van Senten JR  et al. Use of CRISPR/Cas9-edited HEK293 cells reveals that both conventional and novel protein kinase C isozymes are involved in mGlu5a receptor internalization. J Biol Chem 298:102466 (2022). PubMed: 36087841
  • Perveen K  et al. Characterization of the Transient Deficiency of PKC Isozyme Levels in Immature Cord Blood T Cells and Its Connection to Anti-Allergic Cytokine Profiles of the Matured Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34884454
  • Zeng H  et al. Activated PKB/GSK-3ß synergizes with PKC-d signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I. Acta Pharm Sin B 11:71-88 (2021). PubMed: 33532181
  • Pohjolainen L  et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253
  • Liu HY  et al. Protein Kinase C-? Deficiency Does Not Impair Antiviral Immunity and CD8+ T Cell Activation. J Immunol 204:2439-2446 (2020). PubMed: 32198145

レビューと Q&A

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Flow Cytometry abreview for Anti-PKC eta antibody [EPR18513]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (T cells)
Permeabilization
Yes - Perm Buffer (ebiosciences)
Specification
T cells
Fixation
Fixation buffer (BD Cytofix)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Sep 15 2017

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