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リコンビナントRabMAb

Anti-PKC delta (phospho Y311) 抗体 [EPR2609Y] (ab76181)

Reviews (2) Submit a question References (7)
Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
  • Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
  • Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
  • Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
  • Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311)
  • Suitable for: Dot blot, WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-PKC delta (phospho Y311) antibody [EPR2609Y]
    PKC delta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa cell lysate treated with Phorbol-12-myristate-13-acetate and U937 cell lysate treated with TPA. IHC-P: Human urinary bladder carcinoma tissue. IP: HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76181の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
WB (2)
1/2500 - 1/5000. Predicted molecular weight: 77 kDa.
IP
1/40.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Dot blot
1/1000.
WB
1/2500 - 1/5000. Predicted molecular weight: 77 kDa.
IP
1/40.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • ドメイン

    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.

  • 翻訳後修飾

    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.

  • 細胞内局在

    Cytoplasm. Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CVID9 antibody
      • D14Ertd420e antibody
      • Kinase PKC delta antibody
      • KPCD antibody
      • KPCD_HUMAN antibody
      • MAY 1 antibody
      • MAY1 antibody
      • MGC49908 antibody
      • nPKC delta antibody
      • nPKC-delta antibody
      • PCKd antibody
      • PKC d antibody
      • PKC delta antibody
      • PKCD antibody
      • PKCdelta antibody
      • PRKC D antibody
      • PRKC delta antibody
      • Prkcd antibody
      • Protein Kinase C delta antibody
      • Protein kinase C delta type antibody
      • Protein kinase C delta VIII antibody
      • Protein Kinase Cdelta antibody
      • Tyrosine protein kinase PRKCD antibody
      see all

    画像

    • Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/500 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 77 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

      Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

      Exposure time: 3 minutes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

      Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma with ab76181 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

      ab76181 at 1/20 immunoprecipitating PKC delta (phospho Y311) in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.

      Lane 1 (input): HeLa treated with 10mM H2O2 for 1 hour whole cell lysate (10µg)
      Lane 2 (+): ab76181 + HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76181 in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.

      For western blotting, ab76181 was used as a dilution of 1/200 followed by ab131366 VeriBlot for IP (HRP)  at a dilution of 1/1000.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/5000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : U937 cell lysate treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit-HRP at 1/2000 dilution

      Predicted band size: 77 kDa
      Observed band size: 78 kDa why is the actual band size different from the predicted?

    • Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)
      Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181)

    参考文献 (7)

    ab76181 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76181 は 7 報の論文で使用されています。

    • Zhu M  et al. The CCCH-Type Zinc Finger Antiviral Protein Relieves Immunosuppression of T Cells Induced by Avian Leukosis Virus Subgroup J via the NLP-PKC-δ-NFAT Pathway. J Virol 96:e0134421 (2022). PubMed: 34705559
    • Rao Q  et al. Design, Synthesis, and Antileukemic Evaluation of a Novel Mikanolide Derivative Through the Ras/Raf/MEK/ERK Pathway. Front Pharmacol 13:809551 (2022). PubMed: 35721186
    • Nelson LJ  et al. Src Kinase Is Biphosphorylated at Y416/Y527 and Activates the CUB-Domain Containing Protein 1/Protein Kinase C d Pathway in a Subset of Triple-Negative Breast Cancers. Am J Pathol 190:484-502 (2020). PubMed: 31843498
    • Shou X  et al. Baicalin Suppresses Hypoxia-Reoxygenation-Induced Arterial Endothelial Cell Apoptosis via Suppressing PKCd/p53 Signaling. Med Sci Monit 23:6057-6063 (2017). PubMed: 29272263
    • He XJ  et al. Roles of 1,25(OH)2D3 and Vitamin D Receptor in the Pathogenesis of Rheumatoid Arthritis and Systemic Lupus Erythematosus by Regulating the Activation of CD4+ T Cells and the PKCd/ERK Signaling Pathway. Cell Physiol Biochem 40:743-756 (2016). PubMed: 27915349
    • Gruosso T  et al. Chronic oxidative stress promotes H2AX protein degradation and enhances chemosensitivity in breast cancer patients. EMBO Mol Med 8:527-49 (2016). WB . PubMed: 27006338
    • Ficarro SB  et al. Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysis. Mol Cell Proteomics 10:O111.011064 (2011). WB ; Human . PubMed: 21788404

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-PKC delta (phospho Y311) antibody [EP2609Y]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa Cervical Adenocarcinoma, Epithelial)
    Loading amount
    40 µg
    Specification
    HeLa Cervical Adenocarcinoma, Epithelial
    Treatment
    100nM Insulin
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Tomar Ghansah

    Verified customer

    投稿 Feb 20 2013

    Western blot abreview for Anti-PKC delta (phospho Y311) antibody [EP2609Y]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
    Loading amount
    40 µg
    Specification
    C57BL/6 Mouse splenocytes
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Tomar Ghansah

    Verified customer

    投稿 Oct 09 2012

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