Anti-PKC delta (phospho T505) 抗体 (ab60992)
Key features and details
- Rabbit polyclonal to PKC delta (phospho T505)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC delta (phospho T505) antibody
PKC delta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC delta (phospho T505) -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human PKC delta (internal sequence) (phospho T505).
Database link: Q05655 -
ポジティブ･コントロール
- WB: Extracts from NIH/3T3cells, treated with UV (15mins); C57BL/6 Mouse splenocytes cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab60992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
|
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| WB | (2) |
1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
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| 特記事項 |
|---|
|
IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). |
ターゲット情報
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機能
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
ドメイン
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner. -
翻訳後修飾
Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5580 Human
- Entrez Gene: 18753 Mouse
- Entrez Gene: 170538 Rat
- Omim: 176977 Human
- SwissProt: Q05655 Human
- SwissProt: P28867 Mouse
- SwissProt: P09215 Rat
- Unigene: 155342 Human
see all -
別名
- CVID9 antibody
- D14Ertd420e antibody
- Kinase PKC delta antibody
see all
画像
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Western blot - Anti-PKC delta (phospho T505) antibody (ab60992)All lanes : Anti-PKC delta (phospho T505) antibody (ab60992) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3cells,
treated with UV (15mins).
Lane 2 : Extracts from NIH/3T3cells,
treated with UV (15mins) with immunizing phosphopeptide.
Predicted band size: 77 kDa
Observed band size: 77 kDa -
Western blot - Anti-PKC delta (phospho T505) antibody (ab60992)Anti-PKC delta (phospho T505) antibody (ab60992) at 1/500 dilution + C57BL/6 Mouse splenocytes cells at 40 µg
Secondary
HRP Anti-rabbit IgG Goat (H&L) at 1/1000 dilution
Predicted band size: 77 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho T505) antibody (ab60992)ab60992 staining human normal tonsil tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab60992 は 2 報の論文で使用されています。
- Feng L et al. Hepatic Knockdown of Endothelin Type A Receptor (ETAR) Ameliorates Hepatic Insulin Resistance and Hyperglycemia Through Suppressing p66Shc-Mediated Mitochondrial Fragmentation in High-Fat Diet-Fed Mice. Diabetes Metab Syndr Obes 14:963-981 (2021). PubMed: 33688230
- Lee PC et al. Targeting PKCd as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell 34:954-969.e4 (2018). PubMed: 30537515