Anti-PKC beta 1 (phospho T642) 抗体 (ab5782)
Key features and details
- Rabbit polyclonal to PKC beta 1 (phospho T642)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC beta 1 (phospho T642) antibody
PKC beta 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC beta 1 (phospho T642) -
由来種
Rabbit -
特異性
This antibody does not react with PKC beta 2 [pT641], alpha [pT638], nu [pT655], epsilon [pT710], iota [pT555], eta [pT655], or zeta [pT560] as determined by peptide competition experiments. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to PKC beta 1 (phospho T642).
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ポジティブ･コントロール
- K562 cells treated with PMA, a phorbol ester.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC beta 1. The final product is generated by affinity chromatography using a PKC beta 1-derived peptide that is phosphorylated at threonine 642. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5782の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 80 kDa.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 80 kDa. |
ターゲット情報
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機能
Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. -
細胞内局在
Cytoplasm. Nucleus. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5579 Human
- Omim: 176970 Human
- SwissProt: P05771 Human
- Unigene: 460355 Human
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別名
- KPCB_HUMAN antibody
- PKC beta antibody
- PKC-B antibody
see all
画像
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Western blot - Anti-PKC beta 1 (phospho T642) antibody (ab5782)
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (ë) phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5782 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method.
The data show that only the peptide corresponding to PKC beta I [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (ë) phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5782 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta I [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab5782 は 2 報の論文で使用されています。
- El-Shewy HM et al. Phospholipase C and Protein Kinase C-ß 2 Mediate Insulin-Like Growth Factor II-Dependent Sphingosine Kinase 1 Activation. Mol Endocrinol 25:2144-56 (2011). WB ; Human . PubMed: 22016563
- Hsieh TJ et al. Upregulation of osteopontin gene expression in diabetic rat proximal tubular cells revealed by microarray profiling. Kidney Int 69:1005-15 (2006). PubMed: 16528250